Glucose challenge stimulates reactive oxygen species (ROS) generation by leucocytes
Glucose challenge stimulates reactive oxygen species (ROS) generation by leucocytes
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DOI：
10.1210/jc.85.8.2970
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发表时间：
2000-08-01
影响因子：
5.8
通讯作者：
Dandona, P
中科院分区：
文献类型：
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作者：
Mohanty, P;Hamouda, W;Dandona, P
糖尿病与ROS生成增加、氧化损伤和肥胖有关。为了阐明营养与ROS生成之间的关系，我们研究了葡萄糖刺激对白细胞、p47（phox）蛋白（NADPH氧化酶的关键蛋白）和α -生育酚水平产生ROS的影响。14名正常受试者在摄入75 g葡萄糖前、1、2和3小时采集血样。多形核白细胞（PMNL）和单核细胞（MNC）的ROS生成在2h时分别达到峰值244 +/- 42%和233 +/- 34%。葡萄糖摄入后2 h和3 h， MNC匀浆中p47（phox）水平显著升高。α -生育酚水平在1小时、2小时和3小时显著下降。我们得出结论，葡萄糖摄入刺激ROS生成和NADPH氧化酶的p47(phox)；增加氧化负荷，导致α -生育酚浓度下降。
Diabetes mellitus is associated with increased ROS generation, oxidative injury and obesity. To elucidate the relationship between nutrition and ROS generation, we have investigated the effect of glucose challenge on ROS generation by leucocytes, p47(phox) protein, a key protein in the enzyme NADPH oxidase and alpha -tocopherol levels. Blood samples were drawn from 14 normal subjects prior to, at 1, 2 and 3 h following ingestion of 75 g glucose. ROS generation by polymorphonuclear leucocytes (PMNL) and mononuclear cells (MNC) increased to a peak of 244 +/- 42% and 233 +/- 34% of the basal respectively at 2h. The levels of p47(phox) in MNC homogenates increased significantly at 2 h and 3 h after glucose intake. alpha -Tocopherol levels decreased significantly at 1 h, 2 h and 3 h. We conclude that glucose intake stimulates ROS generation and p47(phox) of NADPH oxidase; increases oxidative load and causes a fall in alpha -tocopherol concentration.