Systematic comparison of biologically active foreign ions-codoped calcium phosphate microparticles on osteogenic differentiation in rat osteoporotic and normal mesenchymal stem cells.
Systematic comparison of biologically active foreign ions-codoped calcium phosphate microparticles on osteogenic differentiation in rat osteoporotic and normal mesenchymal stem cells.
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DOI：
10.18632/oncotarget.16618
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发表时间：
2017-05-30
期刊：
影响因子：
--
通讯作者：
Zou H
中科院分区：
文献类型：
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作者：
Chen XY;Xu SZ;Wang XW;Yang XY;Ma L;Zhang L;Yang GJ;Yang F;Wang LH;Zhang XL;Ting K;Gao CY;Mou XZ;Gou ZR;Zou H
骨质疏松症是一种以骨组织结构恶化为特征的疾病，导致骨骼脆性并增加骨折风险。磷酸钙被广泛应用于骨组织工程策略中，因为它们与骨磷灰石有相似之处，除了在骨组织工程中缺乏微量元素(TES)。在过去的二十年里，生物活性玻璃(BGS)也被成功地应用于颅颌面部和牙科的临床应用，因为它具有与骨结合和诱导成骨细胞分化的良好潜力。本研究采用四甲基偶氮唑盐比色法和茜素红S染色方法，研究了四元共掺硅锶锌镁共掺钙盐(TES-CAP)和45S5 Bioglass®(45S5 BG)的离子溶解产物对骨质疏松和正常大鼠骨髓间充质干细胞(rOMSCs和rMSCs)的成骨作用。材料促进细胞增殖和成骨分化，尤其是BG和TES-CAP的结合。定量聚合酶链式反应和酶联免疫吸附试验分析表明，成骨特异性基因和蛋白的表达上调。这些研究表明，TES-CAP和45S5 BG协同作用，创造了促进骨质疏松和正常rMSCs增殖和终末成骨分化的细胞外环境。
Osteoporosis is a disease characterized by structural deterioration of bone tissue, leading to skeletal fragility with increased fracture risk. Calcium phosphates (CaPs) are widely used in bone tissue engineering strategies as they have similarities to bone apatite except for the absence of trace elements (TEs) in the CaPs. Bioactive glasses (BGs) have also been used successfully in clinic for craniomaxillofacial and dental applications during the last two decades due to their excellent potential for bonding with bone and inducing osteoblastic differentiation. In this study, we evaluated the osteogenic effects of the ionic dissolution products of the quaternary Si-Sr-Zn-Mg-codoped CaP (TEs-CaP) or 45S5 Bioglass® (45S5 BG), both as mixtures and separately, on rat bone marrow-derived mesenchymal stem cells (rOMSCs & rMSCs) from osteoporotic and normal animals, using an MTT test and Alizarin Red S staining. The materials enhanced cell proliferation and osteogenic differentiation, especially the combination of the BG and TEs-CaP. Analysis by quantitative PCR and ELISA indicated that the expression of osteogenic-specific genes and proteins were elevated. These investigations suggest that the TEs-CaP and 45S5 BG operate synergistically to create an extracellular environment that promotes proliferation and terminal osteogenic differentiation of both osteoporotic and normal rMSCs.