One- and two-photon excited optical pH probing for cells using surface-enhanced Raman and hyper-Raman nanosensors
One- and two-photon excited optical pH probing for cells using surface-enhanced Raman and hyper-Raman nanosensors
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DOI：
10.1021/nl071418z
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发表时间：
2007-09-01
期刊：
影响因子：
10.8
通讯作者：
Kneipp, Katrin
中科院分区：
文献类型：
--
作者：
Kneipp, Janina;Kneipp, Harald;Kneipp, Katrin
我们证明了空间分辨探测和成像的pH值在活细胞的移动的和生物相容性纳米传感器使用表面增强拉曼散射（Sers）的4-巯基苯甲酸（pMBA）的金纳米聚集体。此外，我们还表明，这一概念的pH值纳米传感器可以扩展到双光子激发，通过使用表面增强超拉曼散射（SEHRS）。除了双光子激发的优点外，SEHRS传感器还可以在宽pH范围内进行测量，而无需使用多个探针。
We demonstrate spatially resolved probing and imaging of pH in live cells by mobile and biocompatible nanosensors using surface-enhanced Raman scattering (SERS) of 4-mercaptobenzoic acid (pMBA) on gold nanoaggregates. Moreover, we also show that this concept of pH nanosensors can be extended to two-photon excitation by using surface-enhanced hyper-Raman scattering (SEHRS). In addition to the advantages of two-photon excitation, the SEHRS sensor enables measurements over a wide pH range without the use of multiple probes.