Enzyme-assisted extraction of flavonoids from Ginkgo biloba leaves: Improvement effect of flavonol transglycosylation catalyzed by Penicillium decumbens cellulase
Enzyme-assisted extraction of flavonoids from Ginkgo biloba leaves: Improvement effect of flavonol transglycosylation catalyzed by Penicillium decumbens cellulase
复制标题
DOI：
10.1016/j.enzmictec.2010.09.017
复制
发表时间：
2011-01-05
影响因子：
3.4
通讯作者：
Xu, Ming-Shu
中科院分区：
文献类型：
--
作者：
Chen, Shuo;Xing, Xin-Hui;Xu, Ming-Shu
我们报道了一种新的酶参与的方法来改进银杏叶中黄酮类化合物的提取，在该方法中，该酶不仅用于降解细胞壁，而且还用于增加目标化合物在乙醇-水萃取剂中的溶解度。德肯青霉纤维素酶是一种商品化的细胞壁降解酶，具有很高的糖基化活性，在麦芽糖作为糖基供体的情况下，其提取效果远远好于里氏木霉纤维素酶和黑曲霉果胶酶。TLC、HPLC和MS分析表明，该纤维素酶能将黄酮苷元糖基转移为更多的极性糖苷，其溶解度越高，提取效果越好。考察了糖基供体、pH、溶剂和温度对酶促糖基化反应的影响。对于银杏中的三种主要黄酮类化合物，糖基化反应的最佳条件相似，因此可用于酶辅助提取。萃取率为28.3 mg/g干重，比优化前提高了31%，比不加酶时提高了102%。本文所描述的酶双功能的利用，命名为目标化合物的酶修饰和促进细胞壁的降解，为从植物中提取天然化合物提供了一种新的方法。(C)2010 Elsevier Inc.保留所有权利。
We report a novel enzyme-involved approach to improve the extraction of flavonoids from Ginkgo biloba, in which the enzyme is employed not only for cell wall degradation, but also for increasing the solubility of target compounds in the ethanol-water extractant. Penicillium decumbens cellulase, a commercial cell wall-degrading enzyme with high transglycosylation activity, was found to offer far better performance in the extraction than Trichoderma reesei cellulase and Aspergillus niger pectinase under the presence of maltose as the glycosyl donor. TLC, HPLC and MS analysis indicated that P. decumbens cellulase could transglycosylate flavonol aglycones into more polar glucosides, the higher solubility of which led to improved extraction. The influence of glycosyl donor, pH, solvent and temperature on the enzymatic transglycosylation was investigated. For three predominant flavonoids in G. biloba, the transglycosylation showed similar optimal conditions, which were therefore used for the enzyme-assisted extraction. The extraction yield turned to be 28.3 mg/g of dw, 31% higher than that under the pre-optimized conditions, and 102% higher than that under the conditions without enzymes. The utilization of enzymatic bifunctionality described here, naming enzymatic modification of target compounds and facilitation of cell wall degradation, provides a novel approach for the extraction of natural compounds from plants. (C) 2010 Elsevier Inc. All rights reserved.