Preparation and antiherpetic activities of chemically modified polysaccharides from Polygonatum cyrtonema Hua
Preparation and antiherpetic activities of chemically modified polysaccharides from Polygonatum cyrtonema Hua
复制标题
化学修饰黄精多糖的制备及其抗疱疹活性
DOI：
10.1016/j.carbpol.2010.08.044
复制
发表时间：
2011-01-10
影响因子：
11.2
通讯作者：
Lu, Xiao-xia
中科院分区：
文献类型：
--
作者：
Liu, Xiao-xiao;Wan, Zhen-jiang;Lu, Xiao-xia
以黄精中性多糖（PD）为原料，制备了磺化、磷酸化、羧甲基化、乙酰化、磺化等化学修饰多糖，并用FT-IR H-1 NMR和C-13 NMR对化合物进行了表征，并利用细胞病变效应评价了天然多糖和修饰后的黄精多糖对环磷酰胺（CP）诱导的单纯疱疹病毒（HSV）的抗疱疹活性（CPE）抑制实验结果表明，与天然衍生物相比，磷酰化衍生物（P-PD）和硫酰化衍生物（S-PD）对HSV具有显著的抑制活性，特别是P-PD具有更强的抗病毒能力，磺化衍生物（Ts-PD）的抗病毒效果与PD相当，乙酰化衍生物（Ac-PD）的抗病毒效果略差，而羧甲基化衍生物（C-PD）的抗病毒效果几乎为无活性功能基团的类型似乎对多糖的抗疱疹活性非常重要(C) 2010 Elsevier Ltd All rights reserved
The chemically modified polysaccharides including sulfated phosphorylated carboxymethylated acetylated and sulfonylated derivatives were prepared from a neutral polysaccharide (PD) extracted from Polygonatum cyrtonema Hua These compounds were characterized by FT-IR H-1 NMR and C-13 NMR spectroscopy Antiherpetic activities of natural and modified P cyrtonema polysaccharide against herpes simplex virus (HSV) Induced by cyclophosphamide (CP) were then evaluated on vero cells using cytopathic effect (CPE) inhibition assay The phorphorylated derivative (P-PD) and sulfated derivative (S-PD) exhibited significant inhibitory activity against HSV in comparison with the native ones especially P-PD presented better antiviral potency In addition the sulfonylated derivative (Ts-PD) was found to be as effective as PD and the acetylated derivative (Ac-PD) to be slightly less antiviral effective whereas carboxymethylated derivative (C-PD) was shown to be almost inactive The results indicated that the types of function groups appeared to be very important for the antiherpetic activity of polysaccharides (C) 2010 Elsevier Ltd All rights reserved