A family of fibrinogen-related proteins that precipitates parasite-derived molecules is produced by an invertebrate after infection
A family of fibrinogen-related proteins that precipitates parasite-derived molecules is produced by an invertebrate after infection
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DOI：
10.1073/pnas.94.16.8691
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发表时间：
1997-08-05
影响因子：
11.1
通讯作者：
Loker, ES
中科院分区：
文献类型：
--
作者：
Adema, CM;Hertel, LA;Loker, ES
双脐螺感染双殖吸虫后，血淋巴中的蛋白质含量明显降低。glabrata含有由65-kDa亚基组成的凝集素，其沉淀由E.在未感染的蜗牛的血淋巴中缺乏类似的活性。获得了与来自65-kDa凝集素的肽具有序列相似性的三种不同cDNA，并且意外地发现其编码纤维蛋白原相关蛋白（FREPs）。这些FREP还含有与IG超家族成员B具有序列相似性的区域。glabrata具有至少五个FREP基因，其中三个在感染后以增加的水平表达。阐明了B的防御系统的组分。glabrata是相关的，因为这种蜗牛是曼氏血吸虫（Schistosoma mansoni）的中间宿主，曼氏血吸虫是人类血吸虫病最广泛分布的病原体。这些结果是新的无脊椎动物的FREPs在识别寄生虫衍生的分子的作用，也提供了一个模型，用于调查的多样性的分子功能在非自我识别的无脊椎动物。
After infection with the digenetic trematode Echinostoma paraensei, hemolymph of the snail Biomphalaria. glabrata contains lectins comprised of 65-kDa subunits that precipitate polypeptides secreted by E. paraensei intramolluscan larvae, Comparable activity is lacking in hemolymph of uninfected snails. Three different cDNAs with sequence similarities to peptides derived from the 65-kDa lectins were obtained and unexpectedly found to encode fibrinogen-related proteins (FREPs). These FREPs also contained regions with sequence similarity to Ig superfamily members, B. glabrata has at least five FREP genes, three of which are expressed at increased levels after infection, Elucidation of components of the defense system of B. glabrata is relevant because this snail is an intermediate host for Schistosoma mansoni, the most widely distributed causative agent of human schistosomiasis. These results are novel in suggesting a role for invertebrate FREPs in recognition of parasite-derived molecules and also provide a model for investigating the diversity of molecules functioning in nonself-recognition in an invertebrate.