Synthetic molecules: helping to unravel plant signal transduction
Synthetic molecules: helping to unravel plant signal transduction
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DOI：
10.1007/s12154-013-0091-8
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发表时间：
2013-03
期刊：
影响因子：
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通讯作者：
Wei Xuan;Evan Murphy;T. Beeckman;D. Audenaert;Ive De Smet
中科院分区：
文献类型：
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作者：
Wei Xuan;Evan Murphy;T. Beeckman;D. Audenaert;Ive De Smet
小分子的应用在鉴定参与植物信号传导的新组分方面发挥了至关重要的作用。与经典的遗传方法相比，小分子筛选在解剖植物生物过程中提供了显着的优势，例如技术简单，启动成本低，最重要的是，绕过了致命性和冗余的问题。为了鉴定靶向生物过程或感兴趣蛋白质的小分子，稳健且合理的高通量筛选方法至关重要。本文综述了拟南芥小分子筛选的原理和方法。我们还提供了一个小分子的概述，导致在发现植物激素信号通路，内膜信号级联，新的生长调节剂和植物防御机制的突破。同时，对这些小分子药物的抗肿瘤作用机制进行了探讨.此外，小分子应用在翻译生物学的机遇和挑战进行了讨论。
The application of small molecules has played a crucial role in identifying novel components involved in plant signalling. Compared to classic genetic approaches, small molecule screens offer notable advantages in dissecting plant biological processes, such as technical simplicity, low start-up costs, and most importantly, bypassing the problems of lethality and redundancy. To identify small molecules that target a biological process or protein of interest, robust and well-reasoned high-throughput screening approaches are essential. In this review, we present a series of principles and valuable approaches in small molecule screening in the plant model systemArabidopsis thaliana. We also provide an overview of small molecules that led to breakthroughs in uncovering phytohormone signalling pathways, endomembrane signalling cascades, novel growth regulators, and plant defence mechanisms. Meanwhile, the strategies to deciphering the mechanisms of these small molecules onArabidopsisare highlighted. Moreover, the opportunities and challenges of small molecule applications in translational biology are discussed.