A quantitative trait locus, qSE3, promotes seed germination and seedling establishment under salinity stress in rice
A quantitative trait locus, qSE3, promotes seed germination and seedling establishment under salinity stress in rice
复制标题
数量性状基因座 qSE3 在盐胁迫下促进水稻种子萌发和幼苗建立
DOI：
10.1111/tpj.14181
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发表时间：
2019-03-01
期刊：
影响因子：
7.2
通讯作者：
Wang, Zhoufei
中科院分区：
文献类型：
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作者：
He, Yongqi;Yang, Bin;Wang, Zhoufei
种子萌发是一个由数量性状位点和环境因子共同决定的复杂性状。本研究定位了水稻盐胁迫下种子萌发和成苗的主要QTL qSE3。为了了解该QTL的分子基础，我们通过基于图谱的克隆分离出qSE3，发现它编码一个K+转运基因OsHAK21。盐度胁迫使萌发种子中qSE3的表达显著上调。生理分析表明，qSE3显著增加了盐胁迫下萌发种子对K+和Na+的吸收，增加了ABA的生物合成，激活了ABA信号响应。此外，qSE3显著降低了盐度胁迫下萌发种子的H2O2水平。这些生理变化可能与盐胁迫下种子萌发和成苗有关。通过对水稻材料单核苷酸多态性数据的分析，我们发现qSE3的HAP3单倍型与盐胁迫下种子萌发呈正相关。本研究为qSE3在盐胁迫下种子萌发和成苗过程中的作用提供了重要的认识，为qSE3在水稻育种中的实际应用提供了依据。
Summary Seed germination is a complex trait determined by both quantitative trait loci (QTLs) and environmental factors and also their interactions. In this study, we mapped one major QTL qSE3 for seed germination and seedling establishment under salinity stress in rice. To understand the molecular basis of this QTL, we isolated qSE3 by map‐based cloning and found that it encodes a K+ transporter gene, OsHAK21. The expression of qSE3 was significantly upregulated by salinity stress in germinating seeds. Physiological analysis suggested that qSE3 significantly increased K+ and Na+ uptake in germinating seeds under salinity stress, resulting in increased abscisic acid (ABA) biosynthesis and activated ABA signaling responses. Furthermore, qSE3 significantly decreased the H2O2 level in germinating seeds under salinity stress. All of these seed physiological changes modulated by qSE3 might contribute to seed germination and seedling establishment under salinity stress. Based on analysis of single‐nucleotide polymorphism data of rice accessions, we identified a HAP3 haplotype of qSE3 that was positively correlated with seed germination under salinity stress. This study provides important insights into the roles of qSE3 in seed germination and seedling establishment under salinity stress and facilitates the practical use of qSE3 in rice breeding.