Resolution of Cell Fate Decisions Revealed by Single-Cell Gene Expression Analysis from Zygote to Blastocyst
Resolution of Cell Fate Decisions Revealed by Single-Cell Gene Expression Analysis from Zygote to Blastocyst
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DOI：
10.1016/j.devcel.2010.02.012
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发表时间：
2010-04-20
影响因子：
11.8
通讯作者：
Robson, Paul
中科院分区：
文献类型：
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作者：
Guo, Guoji;Huss, Mikael;Robson, Paul
三种不同的细胞类型存在于64细胞期小鼠囊胚内。我们已经研究了细胞发育到这个阶段，使用超过500个细胞的单细胞表达分析。分析的48个基因部分是基于对800多个转录因子的全胚胎分析而选择的。我们表明，在桑葚胚中，卵裂球共表达不同谱系特异性的转录因子，但到了64细胞阶段，可以根据其定量表达谱清楚地区分三种细胞类型。我们确定Id 2和Sox 2作为最早的标记外和内细胞，分别。随后是早期内细胞团中受体-配体对Fgfr 2/Fgf 4表达的负相关。位置和信号事件似乎先于转录程序的成熟。这些结果说明了单细胞表达分析的力量，以提供深入了解发育机制。该技术可广泛应用于其他生物系统。
Three distinct cell types are present within the 64-cell stage mouse blastocyst. We have investigated cellular development up to this stage using single-cell expression analysis of more than 500 cells. The 48 genes analyzed were selected in part based on a whole-embryo analysis of more than 800 transcription factors. We show that in the morula, blastomeres co-express transcription factors specific to different lineages, but by the 64-cell stage three cell types can be clearly distinguished according to their quantitative expression profiles. We identify Id2 and Sox2 as the earliest markers of outer and inner cells, respectively. This is followed by an inverse correlation in expression for the receptor-ligand pair Fgfr2/Fgf4 in the early inner cell mass. Position and signaling events appear to precede the maturation of the transcriptional program. These results illustrate the power of single-cell expression analysis to provide insight into developmental mechanisms. The technique should be widely applicable to other biological systems.