C-13 NMR cross polarization and magic angle spinning (CPMAS) and gas chromatography mass spectrometry analysis of the products from a soda pulp mill effluent decolourised by two Streptomyces strains
C-13 NMR cross polarization and magic angle spinning (CPMAS) and gas chromatography mass spectrometry analysis of the products from a soda pulp mill effluent decolourised by two Streptomyces strains
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DOI：
10.1007/s002530050926
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发表时间：
1997-03-01
影响因子：
5
通讯作者：
Arias, ME
中科院分区：
文献类型：
--
作者：
Hernandez, M;Rodriguez, J;Arias, ME
两株链霉菌UAH 30和UAH 51对麦草半化学碱法制浆后的造纸废水具有脱色作用。对菌株UAH 30和UAH 51脱色后的废水进行分级，结果表明，在生长7天后，分别有60%和80%的碱木质素级分从废水中去除。脱色后流出物中剩余的碱木质素的C-13 NMR交叉极化和魔角旋转（CPMAS）光谱显示，与从未处理的流出物获得的相比，芳香族木质素单元的相对量减少，沿着有异戊烯基：愈创木基单元的比率减少。从脱色的流出物中提取的低分子量化合物的气相色谱/质谱分析揭示了未处理的对照流出物中不存在的新的芳香族木质素相关化合物的存在。这与两种链霉菌菌株在脱色过程中发生的较大木质素分子的一般解聚有关。从脱色的流出物中提取的低分子量的芳香族化合物的鉴定显示，在由菌株UAH 30脱色的流出物中仅存在对羟基苯基单元，而在由链霉菌菌株UAH 51脱色的流出物中检测到对羟基苯基、愈创木基和双辛基单元。该研究表明，脱色是两种链霉菌菌株的共同特征，但木质素组分的降解机制可能不同，并且具有菌株特异性。
Two Strepfomyces strains, UAH 30 and UAH 51, have been shown to decolourise a paper-mill effluent obtained after semichemical alkaline pulping of wheat straw. Fractionation of the effluent decolourised by strains UAH 30 and UAH 51 showed that 60% and 80% respectively of the alkali-lignin fraction have been removed from the effluent after 7 days of growth. C-13 NMR cross polarization and magic angle spinning (CPMAS) spectra of the alkali-lignin remaining in the effluent after decolourisation revealed a decrease in the relative amount of aromatic lignin units compared to that obtained from the untreated effluent along with a reduction in the ratio of syringyl:guaiacyl units. Gas chromatography/mass spectrometry analysis of the low-molecular-mass compounds extracted from the decolourised effluent revealed the presence of new aromatic lignin-related compounds that were not present in the untreated control effluent. This was linked to a general depolymerization of larger lignin molecules occurring during decolourisation by the two Streptomyces strains. Identification of low-molecular-mass aromatic compounds extracted from the decolourised effluent revealed only the presence of p-hydroxyphenyl units in effluents decolourised by the strain UAH 30 while p-hydroxyphenyl, guaiacyl and syringyl units were detected in effluents decolourised by Streptomyces strain UAH 51. The study indicates that, while decolourisation is a common feature of the two Streptomyces strains, the mechanisms involved in the degradation of the lignin fractions may be different and strain-specific.