A proteomic profile of washing fluid from the colorectal tract to search for potential biomarkers of colon cancer
A proteomic profile of washing fluid from the colorectal tract to search for potential biomarkers of colon cancer
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DOI：
10.1039/c2mb05394b
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发表时间：
2012-01-01
影响因子：
--
通讯作者：
Lucacchini, Antonio
中科院分区：
文献类型：
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作者：
Giusti, Laura;Iacconi, Pietro;Lucacchini, Antonio
手术切除后结肠直肠道的冲洗液（WF）可能是获得来自肿瘤上皮细胞分泌的蛋白质混合物的第一步，这些蛋白质可能参与组织的病理进展。在这项研究中，我们进行了蛋白质组学分析结直肠WF，以寻找潜在的生物标志物的结肠癌。这种方法的结果可能会打开使用WF筛查结直肠癌（CRC）的癌前和早期阶段的可能性。在手术期间从35名因可疑腺癌或癌而接受结肠切除术的患者获得WF样品，而通过洗涤健康切片获得相应的对照。将WF立即离心，浓缩并加入三氯乙酸（TCA）以获得蛋白质沉淀。经双向凝胶电泳（2DE）后，将恶性肿瘤标本与相应正常标本的蛋白质图谱进行比较。发现41个蛋白质点是差异表达的，表现出>=平均值点强度的倍数变化。经过质谱分析，这些蛋白质点分解成38种不同的蛋白质。有趣的是，在研究中鉴定的19种差异表达蛋白对应于那些被认为是CRC的潜在生物标志物的蛋白。根据文献，这些蛋白质显示出相同的变化方向（所有蛋白质都是向上或向下）。我们的研究结果表明，WF有潜力成为一种探索临床样本的生物标志物和药物靶点发现的方法。
Washing fluid (WF) from the colon rectal tract after surgical resection might represent a first step in obtaining a mixture of proteins derived from the secretion of tumoral epithelial cells potentially involved in the pathological progression of tissue. In this study, we performed a proteomic analysis of colorectal WF to search for potential biomarkers of colon cancer. The outcome of this approach might open the possibility of using WF to screen for the precancerous and early stages of colorectal cancer (CRC). Samples of WFs were obtained during surgery from 35 patients submitted to colon resection for suspicious adenocarcinoma or carcinoma, while the respective controls were obtained by washing the healthy sections. WFs were immediately centrifuged, concentrated and trichloroacetic acid (TCA) was added to obtain protein pellets. After two-dimensional gel electrophoresis (2DE), the protein patterns of malignant samples were compared with respective normal samples. Forty-one protein spots were found to be differentially expressed exhibiting >= fold-change of mean value spot intensities. After mass spectrometry, these protein spots collapsed into 38 different proteins. Interestingly, 19 of the differentially expressed proteins identified in the study corresponded to those suggested as being potential biomarkers of CRC. In accordance with the literature, these proteins showed the same direction of change (up or down for all proteins). Our results suggest that WF has the potential of being a method for the exploration of clinical samples for biomarker and drug target discovery.