Dispelling the myths - Biocatalysis in industrial synthesis
Dispelling the myths - Biocatalysis in industrial synthesis
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DOI：
10.1126/science.1079237
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发表时间：
2003-03-14
期刊：
影响因子：
56.9
通讯作者：
Wubbolts, MG
中科院分区：
文献类型：
--
作者：
Schoemaker, HE;Mink, D;Wubbolts, MG
生物催化已成为工业合成大宗化学品、医药和农用化学品中间体、活性药物和食品配料的重要工具。然而，应用的数量和多样性是适度的，可能部分是因为生物催化剂的感知或真实的限制，例如有限的酶可用性、底物范围和操作稳定性。最近在基因组学、定向酶进化和生物多样性利用方面的科学突破应该有助于克服这些限制。因此，我们期待许多新的工业应用的生物催化实现，从单步酶转化到定制的多步微生物合成代谢途径工程的手段。
Biocatalysis has emerged as an important tool in the industrial synthesis of bulk chemicals, pharmaceutical and agrochemical intermediates, active pharmaceuticals, and food ingredients. However, the number and diversity of the applications are modest, perhaps in part because of perceived or real limitations of biocatalysts, such as limited enzyme availability, substrate scope, and operational stability. Recent scientific breakthroughs in genomics, directed enzyme evolution, and the exploitation of biodiversity should help to overcome these limitations. As a result, we expect many new industrial applications of biocatalysis to be realized, from single-step enzymatic conversions to customized multistep microbial synthesis by means of metabolic pathway engineering.