Latrunculin B-induced plant dwarfism:: Plant cell elongation is F-actin-dependent
Latrunculin B-induced plant dwarfism:: Plant cell elongation is F-actin-dependent
复制标题
DOI：
10.1006/dbio.2000.0115
复制
发表时间：
2001-03-01
影响因子：
2.7
通讯作者：
Volkmann, D
中科院分区：
文献类型：
--
作者：
Baluska, F;Jasik, J;Volkmann, D
海洋大环内酯类latrunculins是一种高度特异性的毒素，能有效地降解所有真核细胞中的肌动蛋白丝（通常为F-肌动蛋白）。我们表明，latrunculin B是有效的不同类型的细胞在高等植物和描述使用这种药物在探测F-肌动蛋白依赖的生长和植物发育相关的过程。与其他真核生物不同，细胞分裂发生在缺乏所有肌动蛋白丝的植物细胞中。然而，分割平面的对齐经常是扭曲的。除了细胞分裂，胚后发育和形态发生也继续在F-肌动蛋白的情况下。这些实验数据表明，F-肌动蛋白在高等植物的形态发生中的重要性不大，没有F-肌动蛋白，植物或多或少可以正常发育。相比之下，F-肌动蛋白是细胞伸长所必需的。当在萌发过程中加入latrunculin B时，形态正常的拟南芥和黑麦幼苗发育，但由于没有细胞伸长，这些幼苗发育不良，类似于遗传侏儒或环境盆景植物。总之，F-肌动蛋白是必不可少的植物细胞伸长，而这种F-肌动蛋白依赖的细胞伸长是不是一个植物特异性发育程序的基本特征。(C)北京：科学出版社.
Marine macrolides latrunculins are highly specific toxins which effectively depolymerize actin filaments (generally F-actin) in all eukaryotic cells. We show that latrunculin B is effective on diverse cell types in higher plants and describe the use of this drug in probing F-actin-dependent growth and in plant development-related processes. In contrast to other eukaryotic organisms, cell divisions occurs in plant cells devoid of all actin filaments. However, the alignment of the division planes is often distorted. In addition to cell division, postembryonic development and morphogenesis also continue in the absence of F-actin. These experimental data suggest that F-actin is of little importance in the morphogenesis of higher plants, and that plants can develop more or less normally without F-actin. In contrast, F-actin turns out to be essential for cell elongation. When latrunculin B was added during germination, morphologically normal Arabidopsis and rye seedlings developed but, as a result of the absence of cell elongation, these were stunted, resembling either genetic dwarfs or environmental bonsai plants. In conclusion, F-actin is essential for the plant cell elongation, while this F-actin-dependent cell elongation is not an essential feature of plant-specific developmental programs. (C) 2001 Academic Press.