New Insights into BS69 Functions*
New Insights into BS69 Functions*
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DOI：
10.1074/jbc.m600573200
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发表时间：
2006-06
影响因子：
4.8
通讯作者：
Guillaume Velasco;S. Grkovic;S. Ansieau
中科院分区：
文献类型：
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作者：
Guillaume Velasco;S. Grkovic;S. Ansieau
BS69蛋白通常被描述为与各种转录因子相关的共阻遏物。然而，由于缺乏可靠的BS69抗体，该假设主要依赖于标记蛋白的过表达。我们首次提出了一个完整的序列的BS 69和有价值的工具来表征内源性蛋白质。我们发现，全长BS69蛋白，以及次要的选择性剪接亚型，是无处不在的表达，核，并与染色质和有丝分裂染色体。因此，BS69与一组染色质重塑因子相互作用，包括ATP依赖性解旋酶、组蛋白脱乙酰酶和组蛋白甲基转移酶，以及E2F6转录因子。这些数据加强了BS69在基因抑制中的作用，并将BS69与染色质重塑联系起来。
The BS69 protein has been commonly described as a co-repressor associated with various transcription factors. However, this hypothesis relied predominately on overexpression of tagged proteins due to the lack of a reliable BS69 antibody. We present for the first time a complete sequence of BS69 and valuable tools to characterize the endogenous protein. We show that the full-length BS69 protein, as well as minor alternatively spliced isoforms, is ubiquitously expressed, nuclear, and associates with chromatin and mitotic chromosomes. Accordingly, BS69 interacts with a set of chromatin remodeling factors, including ATP-dependent helicases, histone deacetylases, and histone methyltransferases, as well as the E2F6 transcription factor. These data strengthen a role for BS69 in gene repression and link BS69 to chromatin remodeling.