Fine structural localization of endogeneous peroxidase in the endostyle of ascidians, Ciona intestinalis. A part of phylogenetic studies of the thyroid gland.
Fine structural localization of endogeneous peroxidase in the endostyle of ascidians, Ciona intestinalis. A part of phylogenetic studies of the thyroid gland.
复制标题
海鞘内源性过氧化物酶的精细结构定位，海鞘。
DOI：
--
复制
发表时间：
1979
期刊：
影响因子：
--
通讯作者：
F. Sawano
中科院分区：
文献类型：
--
作者：
H. Fujita;F. Sawano
用细胞化学方法在电镜水平上研究了玻璃海鞘内柱过氧化物酶活性的精细结构定位。该器官中只有7区细胞对这种酶活性呈阳性。反应产物在顶端质膜的外表面、粗面内质网的池、核被膜的池、高尔基板层的池以及高尔基区和顶端细胞质的小泡中被识别。通过125I的电镜放射自显影，甲状腺球蛋白样蛋白碘化的主要部位被认为是第7区细胞的顶端质膜区域。不能排除在第7区细胞附近的茎内腔内发生蛋白质碘化的可能性。讨论了过氧化物酶活性的精细结构定位与蛋白质碘化位点之间的关系。
The fine structural localization of peroxidase activity in the endostyle of Ciona intestinalis was studied by cytochemistry at electron microscopic level. Only zone 7 cells in this organ are positive for this enzyme activity. The reaction product is recognized in the external surface of the apical plasma membrane, and in the cisternae of the rough endoplasmic reticulum, of the nuclear envelope, and of the Golgi lamellae, and small vesicles of the Golgi region and of the apical cytoplasm. By electron microscopic autoradiography of 125I, the main site of iodination of thyroglobulin-like protein is considered to be the apical plasma membrane region of the zone 7 cells. The possibility of iodination of protein taking place within the endostylar lumen near the zone 7 cell cannot be ruled out. The relationship between the fine-structural localization of peroxidase activity and the site of iodination of protein is discussed.