Exudates from the wild type and a CR-tolerant strain of Scenedesmus acutus influence differently CR(VI) toxicity to algae

Exudates from the wild type and a CR-tolerant strain of Scenedesmus acutus influence differently CR(VI) toxicity to algae
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野生型和 CR 耐受型尖栅藻菌株的分泌物对藻类的 CR(VI) 毒性影响不同

DOI:
10.1016/s0045-6535(98)00343-9
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发表时间:
1998
期刊:
影响因子:
8.8
通讯作者:
M. Bassi
M. Bassi
中科院分区:
环境科学与生态学2区
文献类型:
--
作者:
M. Corradi;G. orbi;H. Abdel;A. Torelli;M. Bassi

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以野生型(S2-N)和耐铬型(S2-T)的尖栅藻(Scenedesmus acutus)为材料,研究了其分泌物对藻类和大型蚤(Daphnia magna)的减毒作用。在培养基中添加1 mg/l Cr(VI),并用作野生型S.用14 μg/tCr(VI)作为媒介繁殖蚤类。在对藻类的实验中,两者都能部分地抵消铬的生长抑制活性,但S2-T的滤液的效果要大得多。在对水蚤的实验中,藻类寄生虫具有毒性作用,导致生长、繁殖力和寿命降低。同样的效果,获得了与有机质剥夺的植物。S_2-T的蛋白质电泳图谱显示出S_2-N所没有的三个电泳峰,而S_2-T的蛋白质电泳图谱显示出S_2-N所没有的三个电泳峰。
Culture filtrates containing the exudates from the wild type (S2-N) and a Cr-tolerant strain (S2-T) of Scenedesmus acutus were tested for their capacity of reducing Cr toxicity to the algae and the filter-feeder Daphnia magna. The filtrates were supplemented either with 1 mg/1 Cr(VI) and used as culture media for the wild type of S. acutus, or with 14 μg/t Cr(VI) and used as media to breed daphnids. In the experiments with algae both filtrates partially counteracted the growth-inhibiting activity of chromium, but the effect of the filtrate of S2-T was much greater. In the experiments with daphnids the algal filtrates had toxic effects, causing reduction of growth, fecundity and life-span. The same effects were obtained with the filtrates deprived of the organic matter. The filtrates of S2-T differed from those of S2-N because of their higher content in carbohydrates and because the electrophoretic pattern of the proteins extruded by S2-T showed three peaks not present in the proteins extruded by S2-N.