Polymorphisms in GSTT1 and p53 and urinary transitional cell carcinoma in south-western Taiwan:: A Preliminary Study
Polymorphisms in GSTT1 and p53 and urinary transitional cell carcinoma in south-western Taiwan:: A Preliminary Study
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DOI：
10.1080/13547500400010122
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发表时间：
2004-07-01
期刊：
影响因子：
2.6
通讯作者：
Christiani, DC
中科院分区：
文献类型：
--
作者：
Chen, YC;Xu, L;Christiani, DC
遗传多态性与砷相关性膀胱癌的相关性尚不清楚。本研究旨在探讨台湾南部地区原砷高暴露区GSTT1、p53密码子72基因多态性与膀胱癌的关系。本研究于1996年至1999年间，从台湾西南部某转诊中心招募59名尿行性细胞癌（TCC）患者及81名符合居住条件的社区对照者。进行了问卷调查，以获得砷暴露和一般健康信息。采用聚合酶链反应-限制性片段长度聚合酶对p53密码子72和GSTT1进行基因型分析。在29%的病例和44%的对照组中分别观察到p53密码子72和GSTT1缺失的联合变异基因型（杂合或纯合变异）。在本初步研究中，携带p53密码子72变异基因型或携带GSTT1零基因型的受试者膀胱癌风险略有升高。p53密码子72的变异增加了吸烟者患膀胱癌的风险。然而，该结果没有统计学意义，需要更大规模的验证性研究来阐明砷暴露人群中候选基因多态性和膀胱癌风险的作用。
Little is known about the relevance of genetic polymorphisms to arsenic-related bladder cancer. A preliminary case-control study was conducted to explore the association between genetic polymorphisms of GSTT1, p53 codon 72 and bladder cancer in southern Taiwan, a former high arsenic exposure area. Fifty-nine urinary transitional cell carcinoma (TCC) patients from a referral centre in south-western Taiwan and 81 community controls matched on residence were recruited from 1996 to 1999. A questionnaire was administered to obtain arsenic exposure and general health information. Genotypes of p53 codon 72 and GSTT1 were analysed by polymerase chain reaction-restriction fragment length polymerase. The combined variant genotypes (heterozygous or homozygous variant) of p53 codon 72 and GSTT1 null were observed in 29% of cases and in 44% of controls, respectively. In this preliminary study, bladder cancer risk was slightly elevated for subjects carrying the variant genotype of p53 codon 72 or in subjects carrying the GSTT1 null genotype. Variants in p53 codon 72 increased the risk of bladder cancer among smokers. However, the results were not statistically significant and larger confirmatory studies are needed to clarify the role of candidate gene polymorphisms and bladder cancer risk in arsenic exposed populations.