Phylogenetic relationships of Irkut and West Caucasian bat viruses within the Lyssavirus genus and suggested quantitative criteria based on the N gene sequence for lyssavirus genotype definition
Phylogenetic relationships of Irkut and West Caucasian bat viruses within the Lyssavirus genus and suggested quantitative criteria based on the N gene sequence for lyssavirus genotype definition
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DOI：
10.1016/j.virusres.2005.03.008
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发表时间：
2005-07-01
期刊：
影响因子：
5
通讯作者：
Rupprecht, CE
中科院分区：
文献类型：
--
作者：
Kuzmin, IV;Hughes, GJ;Rupprecht, CE
对伊尔库特病毒和西高加索蝙蝠病毒（WCBV）的核蛋白（N）、磷蛋白（P）和糖蛋白（G）基因进行了测序，并与其他狂犬病毒病毒进行了比较。 N 基因核苷酸同一性以 82% 的同一性阈值明确分离了所有狂犬病病毒基因型。在此基础上，伊尔库特病毒应被视为与基因型4和5具有特定相关性的新基因型（N基因核苷酸一致性为78.0-78.6%，氨基酸一致性为90.4-92.6%）。此外，基因型 4-6 与 Aravan、Khujand 和 Irkut 病毒一起构成了旧世界蝙蝠狂犬病病毒的坚实系统群。使用所有三种病毒基因时，这种关系很明显，并导致 P 基因（Aravan、Khujand 病毒和基因型 6）和 G 基因（Aravan、Khujand 病毒和基因型 5 和 6）的基因内型和基因间身份之间的重叠。 WCBV 是已知狂犬病毒中差异最大的一种，与基因型 2 和 3 的相关性有限。(c) 2005 Elsevier B.V. 保留所有权利。
The nucleoprotein (N), phosphoprotein (P) and glycoprotein (G) genes of Irkut and West Caucasian bat viruses (WCBV) were sequenced and compared with those of other lyssaviruses. N gene nucleotide identities provided unequivocal separation of all lyssavirus genotypes with an identity threshold of 82%. On this basis, Irkut virus should be considered as a new genotype with particular relatedness to genotypes 4 and 5 (78.0-78.6% identity for N gene nucleotides and 90.4-92.6% for amino acids). Furthermore, genotypes 4-6, together with Aravan, Khujand and Irkut viruses, present a solid phylogroup of Old World bat lyssaviruses. This relationship is apparent using all three viral genes, and causes overlap between intragenotype and intergenotype identities for the P gene (Aravan, Khujand viruses and genotype 6) and for the G gene (Aravan, Khujand, genotypes 5 and 6). WCBV is the most divergent of known lyssaviruses with only limited relatedness to genotypes 2 and 3. (c) 2005 Elsevier B.V. All rights reserved.