Strong influence of livestock environments on the emergence and dissemination of distinct multidrug-resistant phenotypes among the population of non-typhoidal Salmonella.
Strong influence of livestock environments on the emergence and dissemination of distinct multidrug-resistant phenotypes among the population of non-typhoidal Salmonella.
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DOI：
10.1371/journal.pone.0179005
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发表时间：
2017
期刊：
影响因子：
3.7
通讯作者：
Vidovic S
中科院分区：
文献类型：
--
作者：
An R;Alshalchi S;Breimhurst P;Munoz-Aguayo J;Flores-Figueroa C;Vidovic S
多重耐药性的出现和传播问题，特别是在革兰氏阴性菌中，已经达到令人震惊的程度。这就更加需要开发监测方法，更有效和准确地提供关于耐多药生物体出现和传播的信息。在这项研究中，使用一个明确定义的群体的非伤寒沙门氏菌（NTS）分离株与禽，牛和猪的宿主，我们发现，牲畜的环境有一个特定的（P < 0.005）和深刻的（P < 0.005）的NTS分离株之间的多重耐药表型的演变。含有青霉素类、四环素类和大环内酯类的MDR模式以及不断演变的对应物（即，青霉素类、四环素类和大环内酯类+其他抗生素类）与猪源性NTS分离株显著相关（P < 0.005）。同样，含叶酸途径抑制剂、大环内酯类和氨基环醇或含青霉素类、头孢菌素类、四环素类、酚类和大环内酯类的MDR模式分别与禽类（P < 0.005）和牛（P < 0.005）NTS分离株显著相关。此外，结构，一种进化分析，清楚地表明宿主起源（即，不同NTS血清型的遗传背景是MDR表型获得和传播的最主要决定因素。此外，我们描述了一个新的非同义突变，位于QRDR以外的GyrA的位置864，这可能与氟喹诺酮耐药。
The problem of emergence and dissemination of multidrug resistance, especially among Gram-negative bacteria, has reached alarming levels. This increases the need to develop surveillance methods that more effectively and accurately provide information about the emergence and spread of multidrug-resistant organisms. In this study, using a well-defined population of non-typhoidal Salmonella (NTS) isolates associated with avian, bovine and porcine hosts, we found that the livestock environments had a specific (P < 0.005) and profound (P < 0.005) effect on the evolution of multidrug-resistant phenotypes among population of NTS isolates. The MDR pattern containing penicillins, tetracyclines and macrolides and the evolving counterparts (i.e., penicillins, tetracyclines and macrolides + other antibiotic classes) were significantly (P < 0.005) associated with NTS isolates of porcine origin. Similarly, MDR patterns containing folate pathway inhibitors, macrolides and aminocyclitol or containing penicillins, cephalosporins, tetracyclines, phenicols and macrolides were significantly associated with avian (P < 0.005) and bovine (P < 0.005) NTS isolates, respectively. Furthermore, STRUCTURE, an evolutionary analysis, clearly showed that the host origin (i.e., livestock environment), and not the genetic background of different NTS serovars, was the most determinative factor for acquisition and spread of MDR phenotypes. In addition, we described a novel non-synonymous mutation, located outside of the QRDR at position 864 of GyrA, that was likely associated with fluoroquinolone resistance.