Irregular expression of hyaluronan and its CD44 receptor is associated with metastatic phenotype in laryngeal squamous cell carcinoma
Irregular expression of hyaluronan and its CD44 receptor is associated with metastatic phenotype in laryngeal squamous cell carcinoma
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DOI：
10.1007/s004280050302
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发表时间：
1999-01-01
期刊：
影响因子：
3.5
通讯作者：
Kosma, VM
中科院分区：
文献类型：
--
作者：
Hirvikoski, P;Tammi, R;Kosma, VM
应用软骨蛋白多糖（bHABC）和单克隆抗体（爱马仕3）制备的透明质酸（HA）特异性探针，对24例正常喉上皮、27例喉不典型增生和172例喉鳞状细胞癌（LSCC）组织中HA及其CD 44受体的分布进行了研究。HA和CD 44在所有正常喉上皮和约90%的不典型增生和肿瘤性喉上皮中表达相似。在正常上皮中，HA和CD 44均匀地分布在整个上皮中，而最浅表层为阴性。这与完全HA和CD44阳性的异型增生上皮和分化良好的浸润性癌的情况相反。HA和CD 44低信号的局部区域分别存在于11%和22%的异型增生样本中，以及27%和28%的癌样本中。这种染色不规则性的存在与癌的分化差、有丝分裂指数显著升高以及淋巴结扩散和转移的频率高有关。此外，不规则染色显示无病生存率差的趋势，表明HA代谢改变是LSCC的常见特征，并与侵袭性生长模式相关。
The distributions of hyaluronan (HA) and its CD44 receptor were studied in 24 normal, 27 dysplastic samples of laryngeal epithelium and in 172 squamous cell carcinomas (LSCC), using a specific probe prepared from cartilage proteoglycan (bHABC, biotinylated hyaluronan binding complex) and a monoclonal antibody (Hermes 3). HA and CD44 were expressed similarly in all normal and about 90% of dysplastic and neoplastic laryngeal epithelia. In the normal epithelium HA and CD44 were homogeneously distributed throughout the epithelium, whereas the most superficial layers were negative. This was in contrast to the picture in dysplastic epithelium and well-differentiated invasive carcinomas, which were entirely HA and CD44 positive. Local areas with a low signal for HA and CD44 were present in 11% and 22% of the samples with dysplasia, and in 27% and 28% of those with carcinoma, respectively. The presence of this staining irregularity was associated with poor differentiation of the carcinoma, a significantly elevated mitotic index and a high frequency of nodal spreading and metastases. Furthermore, the irregular staining showed a trend towards poor disease-free survival, suggesting that an altered metabolism of HA is a common feature in LSCC and is associated with an aggressive growth pattern.