ChIP-chip: Data, model, and analysis
ChIP-chip: Data, model, and analysis
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DOI：
10.1111/j.1541-0420.2007.00768.x
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发表时间：
2007-09-01
期刊：
影响因子：
1.9
通讯作者：
Wu, Ying Nian
中科院分区：
文献类型：
--
作者：
Zheng, Ming;Barrera, Leah O.;Wu, Ying Nian
ChIP-chip（或ChIP-on-chip）是一种用于分离和鉴定活细胞中特定DNA结合蛋白所占据的基因组位点的技术。可以通过平铺阵列在整个基因组上获得ChIP芯片信号，其中通常在蛋白质结合位点周围观察到峰形。在这篇文章中，我们描述了ChIP芯片的过程，并提出了一个概率模型的ChIP芯片数据。然后，我们提出了一个基于模型的方法来识别的峰的形状检测蛋白质结合位点的目的。我们还研究了非参数核平滑方法的带宽问题。
ChIP-chip (or ChIP-on-chip) is a technology for isolation and identification of genomic sites occupied by specific DNA-binding proteins in living cells. The ChIP-chip signals can be obtained over the whole genome by tiling arrays, where a peak shape is generally observed around a protein-binding site. In this article, we describe the ChIP-chip process and present a probability model for ChIP-chip data. We then propose a model-based method for recognizing the peak shapes for the purpose of detecting protein-binding sites. We also investigate the issue of bandwidth in nonparametric kernel smoothing method.