THE KARYOGAMY GENE KAR2 AND NOVEL PROTEINS ARE REQUIRED FOR ER-MEMBRANE FUSION
THE KARYOGAMY GENE KAR2 AND NOVEL PROTEINS ARE REQUIRED FOR ER-MEMBRANE FUSION
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DOI：
10.1016/0092-8674(94)90575-4
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发表时间：
1994-07-15
期刊：
影响因子：
64.5
通讯作者：
SCHEKMAN, R
中科院分区：
文献类型：
--
作者：
LATTERICH, M;SCHEKMAN, R
我们已经开发了使用细胞和分离的膜来鉴定介导酵母中ER-核膜网络融合的因子的测定。当在交配过程中观察到含有明显标记的ER-核膜的细胞时，双亲膜的标记物在与核配体共享遗传要求的过程中变得共定位。使用隔离的膜，我们发现ER隔室之间的融合需要ATP，但不是胞质溶胶，Sec 17 p（α-SNAP），或Sec 18 p（NSF），后两个是在囊泡运输的融合步骤所需的。与ER膜紧密结合的蛋白质是融合所必需的，如Kar 2 p（BiP），ER内腔hsp 70同源物。BiP可以激活ER定位的融合子，允许酵母中的核融合和核融合。
We have developed assays using cells and isolated membranes to identify factors mediating fusion of the ER-nuclear membrane network in yeast. When cells containing distinctly tagged ER-nuclear envelops membranes are observed during mating, the markers of both parental membranes become colocalized in a process sharing a genetic requirement with karyogamy. Using isolated membranes, we find that fusion between ER compartments requires ATP, but not cytosol, Sec17p (alpha-SNAP), or Sec18p (NSF), the latter two being required at the fusion step in vesicular transport. Proteins tightly associated with the ER membrane are essential for fusion, as is Kar2p (BiP), an ER lumenal hsp70 homolog. BiP may activate an ER-localized fusogen, allowing nuclear fusion and karyogamy in yeast.