Regional susceptibilities to 12 insecticides of melon and cotton aphid, Aphis gossypii (Hemiptera: Aphididae) and a point mutation associated with imidacloprid resistance
Regional susceptibilities to 12 insecticides of melon and cotton aphid, Aphis gossypii (Hemiptera: Aphididae) and a point mutation associated with imidacloprid resistance
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DOI：
10.1016/j.cropro.2013.09.010
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发表时间：
2014-01-01
期刊：
影响因子：
2.8
通讯作者：
Kim, Gil-Hah
中科院分区：
文献类型：
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作者：
Koo, Hyun-Na;An, Jeong-Jin;Kim, Gil-Hah
棉蚜（Aphis gossypii格洛弗）是世界上危害最严重的害虫之一。调查了A.测定了棉蚜对12种杀虫剂（6种烟碱类、3种拟除虫菊酯类和3种其它）的抗性率。杀虫剂抗性水平极高，尤其是对啶虫脒、噻虫胺、噻虫啉和吡虫啉等烟碱类杀虫剂的抗性。为了确定类烟碱的抗性机制，我们使用了一种吡虫啉抗性（IMI-R）菌株作为模型菌株。IMI-R表现出极高的耐药率和对所有供试类烟碱的交叉耐药。然而，对包括氟啶虫胺腈在内的其他杀虫剂很少或没有交叉抗性。增效试验和酶活性测定表明，缺乏基于增强解毒酶，如细胞色素P450，酯酶和谷胱甘肽S-转移酶的抗性机制。在IMI-R株的烟碱乙酰胆碱受体（nAChR）的β 1亚基环D区发现了一个点突变。在5个地区采集的田间群体中也发现了该R81T点突变。因此，R81T点突变被确定为A.棉花属(C)2013由Elsevier Ltd.出版
The melon and cotton aphid Aphis gossypii Glover (Hemiptera; Aphididae) is one of the most serious pests worldwide. We surveyed insecticide susceptibility in A. gossypii field populations to 12 insecticides (6 neonicotinoids, 3 pyrethroids and 3 others) to examine resistance ratios. The levels of insecticide resistance were extremely high, especially to neonicotinoids, such as acetamiprid, clothianidin, thiacloprid and imidacloprid. To identify the neonicotinoid resistance mechanisms, we used an imidacloprid-resistant (IMI-R) strain as a model strain. IMI-R showed an extremely high resistance ratio and also cross-resistance to all the test neonicotinoids. However, there was little or no cross-resistance to the other insecticides, including sulfoxaflor. Synergist tests and enzyme activity assays suggested the absence of resistance mechanisms based on enhanced detoxification enzymes, such as cytochrome P450, esterase and glutathione S-transferase. One point mutation was found in the beta1 subunit loop D region of the nicotinic acetylcholine receptor (nAChR) of the IMI-R strain. This R81T point mutation was also found in field populations collected from 5 regions. Therefore, the R81T point mutation was identified as an important mechanism of imidacloprid resistance in A. gossypii. (C) 2013 Published by Elsevier Ltd.