Intraocular Irrigating Solutions: Their Effect on the Corneal Endothelium
Intraocular Irrigating Solutions: Their Effect on the Corneal Endothelium
复制标题
眼内冲洗液：对角膜内皮的影响
DOI：
--
复制
发表时间：
1975
期刊：
影响因子：
--
通讯作者：
Richard O. Schultz
中科院分区：
文献类型：
--
作者：
H. Edelhauser;D. L. Horn;R. Hyndiuk;Richard O. Schultz
利用扫描和透射电子显微镜的角膜内皮灌流系统，观察了几种眼内灌注液对兔和猴角膜内皮细胞的影响。经0.9%无菌等渗生理盐水灌流的角膜以60~90μm/小时的速度肿胀，内皮细胞相互分离，表现为广泛的退行性改变。用乳酸林格液灌流的角膜以37μm至40μ/小时的速度膨胀，内皮细胞表现出较慢的进行性变性。用平衡盐液灌流的角膜以24μm/小时至31μm/小时的速度膨胀，退行性变化仅在2小时后变得严重。用含有碳酸氢钠、还原型谷胱甘肽和腺苷的林格液灌流的角膜厚度不会增加，并且在长达6小时的时间内内皮细胞超微结构的恶化最小。
The effects of several intraocular irrigating solutions on the corneal endothelium of rabbit and monkey corneas were evaluated, utilizing a specular microscope perfusion system with both scanning and transmission electron microscopy. Corneas perfused with 0.9% sterile isotonic physiological saline swell at a rate of 60μm to 90μm/hr; endothelial cells separate from each other and show extensive degenerative changes. Corneas perfused with lactated Ringer solution swell at a rate of 37μm to 40μ/hr, and the endothelial cells show slower, but progressive degeneration. Corneas perfused with balanced salt solution swell at 24μm to 31μm/hr, and degenerative changes become severe only after two hours. Corneas perfused with Ringer solution containing bicarbonate, reduced glutathione, and adenosine do not increase in thickness, and there is minimal deterioration of endothelial ultrastructure for periods of up to six hours.