Diagnostic accuracy of ELISA and xMAP technology for analysis of amyloid β42 and Tau proteins
Diagnostic accuracy of ELISA and xMAP technology for analysis of amyloid β42 and Tau proteins
复制标题
DOI：
10.1373/clinchem.2006.081679
复制
发表时间：
2007-05-01
影响因子：
9.3
通讯作者：
Verbeek, Marcel M.
中科院分区：
文献类型：
--
作者：
Reijn, Thierry S. M.;Rikkert, Marcel Olde;Verbeek, Marcel M.
背景资料：脑脊液（CSF）中淀粉样β（42）（A β（42））肽和tau蛋白的浓度可作为阿尔茨海默病（AD）的生物标志物。最近，xMAP技术已被引入作为ELISA的替代品，用于测量这些标志物。方法：我们使用xMAP测定和ELISA分析CSF中A β（42）、总tau（t-tau）和在苏氨酸181处磷酸化的tau的浓度在来自69名阿尔茨海默病患者、26名血管性痴呆患者和55名无神经系统疾病的对照的样本中，对于每种生物标志物，观察到xMAP：ELISA比率的高CV值（> 28%），表明不能应用恒定校正因子将xMAP结果重新计算为ELISA结果。当使用的CSF标志物的组合，灵敏度，特异性，和ROC曲线下的面积xMAP测定和ELISA在区分AD患者血管性痴呆患者和controls.Conclusions：一个恒定的转换因子不能成功地使用重新计算与xMAP测定那些从ELISA获得的结果没有显着差异。然而，通过使用CSF中A β（42）、t-tau和p-tau的组合分析，当使用xMAP技术或传统ELISA时，临床组的区分是等效的。2007年美国临床化学协会
Background: Cerebrospinal fluid (CSF) concentrations of amyloid beta(42) (A beta(42)) peptides and tau proteins may serve as biomarkers for Alzheimer disease (AD). Recently, the xMAP technology has been introduced as an alternative to ELISA for measurement of these markers.Methods: We used xMAP assays and ELISA to analyze CSF concentrations of A beta(42), total tau (t-tau), and tau phosphorylated at threonine 181 (p-tau(181)) in samples from 69 patients with Alzheimer disease, 26 patients with vascular dementia, and 55 controls without neurological disorders.Results: High CV values (> 28%) for the ratio of xMAP: ELISA were observed for each biomarker, indicating that a constant correction factor cannot be applied to recalculate xMAP results into ELISA results. When a combination of CSF markers was used, the sensitivity, specificity, and area under the ROC curves for xMAP assays and ELISAs were not significantly different in differentiating AD patients from vascular dementia patients and controls.Conclusions: A constant conversion factor cannot be used successfully to recalculate results obtained with xMAP assays to those from the ELISAs. With the use of analysis of a combination of A beta(42), t-tau, and p-tau in CSF, however, differentiation of clinical groups is equivalent when either xMAP technology or conventional ELISA is used. 2007 American Association for Clinical Chemistry