Ultrastructural studies of early morphogenesis and cytodifferentiation in the embryonic mammalian pancreas.
Ultrastructural studies of early morphogenesis and cytodifferentiation in the embryonic mammalian pancreas.
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胚胎哺乳动物胰腺早期形态发生和细胞分化的超微结构研究。
DOI：
10.1016/0012-1606(68)90073-0
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发表时间：
1968
影响因子：
2.7
通讯作者：
Jean Evans
中科院分区：
文献类型：
--
作者：
N. K. Wessells;Jean Evans
对小鼠胚胎胰腺的早期形态发生和细胞分化进行了研究。器官形成一个广泛的憩室，其底部变窄，形成导管。在肠道的每个侧壁都发现了新月形的细胞，据信与形态发生有关。讨论了新月体随时间的变化及其细胞的超微结构。提出了一个模型来解释胰腺憩室的抬高，这取决于(1)新月细胞横截面形状的改变和(2)细胞离开新月区的一些相对运动。当这种形态发生过程开始时，可以首先检测到低水平的内分泌和外分泌细胞特异性产物(Rutteret al.，1968)。粗面内质网、高尔基体和各种囊泡出现在推测的外分泌细胞中，但没有发现酶原颗粒。预期的B细胞首先可以通过胞浆电子密度的增加来识别；随后不久就出现了β颗粒。根据生化信息讨论了这些结构的相关性。总而言之，这些数据表明，最初的特定合成和最初的器官形态发生之间存在密切的时间联系。
Early morphogensis and cytodifferentiation of mouse embryonic pancreas have been investigated. The organ forms as a broad-based diverticulum whose base narrows to form the duct. A crescent of cells, believed to be involved in morphogenesis, is found in each lateral wall of the gut. Changes of the crescents with time and the ultrastructure of cells in them is discussed. A model is presented to explain elevation of the pancreatic diverticulum which depends upon (1) alteration in crescent cell cross-sectional shape and (2) upon some relative movement of cells out of the crescent region.Low levels of endocrine and exocrine cell-specific product can first be detected as this morphogenetic process starts (Rutteret al., 1968). Rough endoplasmic reticulum, Golgi apparatus, and various vesicles are seen in presumed exocrine cells at these times, but no prozymogen granules have been found. Prospective B-cells can first be identified by increased cytoplasmic electron density; soon thereafter β-granules appear. These correlations of structure are discussed in light of the biochemical information. All told, the data suggest a close temporal linkage between initial specific synthesis and initial organ morphogenesis.