Electrophoretic separation of influenza virus ribonucleoproteins.

Electrophoretic separation of influenza virus ribonucleoproteins.
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流感病毒核糖核蛋白的电泳分离。

DOI:
10.1099/0022-1317-53-1-125
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发表时间:
1981
期刊:
The Journal of general virology
影响因子:
--
通讯作者:
N. Dimmock
N. Dimmock
中科院分区:
--
文献类型:
--
作者:
P. Rees;N. Dimmock

文献摘要

被引文献

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一种通过聚丙烯酰胺凝胶电泳分离流感病毒颗粒核糖核蛋白(RNP)的新方法可以分辨出五条离散的条带。最大的是含有三个最大RNA(1至3)的三联体,中等大小的RNP是含有RNA 5和6的双联体,其他的每个含有单个RNA。此外,每个RNP由NP蛋白和一定量的M组成,M的量与RNP的大小无关。这表明某些病毒颗粒M与流感病毒核糖核蛋白特异性相关。
A new procedure for the separation of influenza virus particle ribonucleoproteins (RNPs) by electrophoresis on a slab of polyacrylamide gel resolves five discrete bands. The largest is a triplet containing the three largest RNAs (1 to 3), the intermediate sized RNP is a doublet containing RNAs 5 and 6 and the others each contain a single RNA. In addition, each RNP is composed of NP protein and an amount of M which is independent of the size of the RNP. This suggests that some virus particle M is specifically associated with influenza ribonucleoprotein.