Cytotoxic T lymphocytes to Ebola Zaire virus are induced in mice by immunization with liposomes containing lipid A
Cytotoxic T lymphocytes to Ebola Zaire virus are induced in mice by immunization with liposomes containing lipid A
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DOI：
10.1016/s0264-410x(99)00170-x
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发表时间：
1999-08-06
期刊：
影响因子：
5.5
通讯作者：
Alving, CR
中科院分区：
文献类型：
--
作者：
Rao, M;Matyas, GR;Alving, CR
八个氨基酸的序列（TELRTFSI）存在于羧基末端埃博拉病毒的主要结构蛋白膜锚定GP的氨基酸（aa 577-584）被鉴定为H-2（k）特异性鼠细胞毒性T细胞表位，细胞毒性T淋巴细胞（CTL）通过用辐照过的埃博拉病毒或用封装在B10.BR中的辐照过的埃博拉病毒静脉内免疫B10.BR小鼠，含有脂质A的脂质体。经辐照的埃博拉病毒诱导的CTL应答在第二轮用肽体外刺激免疫脾细胞后不能持续，除非将经辐照的病毒包封在含有脂质A的脂质体中。埃博拉GP特异性CTL表位的鉴定和CTL记忆回忆反应对脂质体脂质A的需求可能被证明是开发针对埃博拉病毒感染的疫苗的有前途的方法。由爱思唯尔科技有限公司出版。保留所有权利。
An eight amino acid sequence (TELRTFSI) present in the carboxy terminal end (aa 577-584) of membrane-anchored GP, the major structural protein of Ebola virus, was identified as an H-2(k)-specific murine cytotoxic T cell epitope, Cytotoxic T lymphocytes (CTLs) to this epitope were induced by immunizing B10.BR mice intravenously with either irradiated Ebola virus or with irradiated Ebola virus encapsulated in liposomes containing lipid A. The CTL response induced by irradiated Ebola virus could not be sustained after the second round of in vitro stimulation of immune splenocytes with the peptide, unless the irradiated virus was encapsulated in liposomes containing lipid A. The identification of an Ebola GP-specific CTL epitope and the requirement of liposomal lipid A for CTL memory recall responses could prove to be a promising approach for developing a vaccine against Ebola virus infection. Published by Elsevier Science Ltd. All rights reserved.