Brainstem Stimulation Increases Functional Connectivity of Basal Forebrain-Paralimbic Network in lsoflurane-Anesthetized Rats
Brainstem Stimulation Increases Functional Connectivity of Basal Forebrain-Paralimbic Network in lsoflurane-Anesthetized Rats
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DOI：
10.1089/brain.2014.0254
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发表时间：
2014-09-01
影响因子：
3.4
通讯作者：
Hudetz, Anthony G.
中科院分区：
文献类型：
--
作者：
Pillay, Siveshigan;Liu, Xiping;Hudetz, Anthony G.
大脑状态和认知行为功能由皮质下神经调节网络精确控制。通过脑深部刺激操纵上升觉醒系统(AAS)的关键组成部分，可能有助于促进麻醉动物的全局觉醒。在这里，我们测试了一种假设，即在轻度异氟醚麻醉下，电刺激脑桥网状核(PNO)的口部，与意识丧失相关，导致皮质去同步化和大脑血氧水平依赖(BOLD)的功能连接(FC)的特异性变化。同时采集刺激PNO前后的额部硬膜外脑电BOLD信号。全脑Fc图与AAS主要中枢的种子进行相关分析。刺激PNO导致大脑皮层去同步化，δ和theta频段功率降低，近似熵增加。刺激PNO后，在作为种子的左侧Meynert基底核(NBM)和边缘旁网络的许多区域之间，FC显著增加。丘脑中央内侧核和褶后种子与左侧尾壳核和NBM之间的Fc增加较小。结果提示，在浅麻醉状态下，刺激PNO优先调节基底前脑-边缘旁网络。我们推测，这可能是意识脱节的反映。
Brain states and cognitive-behavioral functions are precisely controlled by subcortical neuromodulatory networks. Manipulating key components of the ascending arousal system (AAS), via deep-brain stimulation, may help facilitate global arousal in anesthetized animals. Here we test the hypothesis that electrical stimulation of the oral part of the pontine reticular nucleus (PnO) under light isoflurane anesthesia, associated with loss of consciousness, leads to cortical desynchronization and specific changes in blood-oxygenation-level-dependent (BOLD) functional connectivity (FC) of the brain. BOLD signals were acquired simultaneously with frontal epidural electroencephalogram before and after PnO stimulation. Whole-brain FC was mapped using correlation analysis with seeds in major centers of the AAS. PnO stimulation produced cortical desynchronization, a decrease in delta- and theta-band power, and an increase in approximate entropy. Significant increases in FC after PnO stimulation occurred between the left nucleus Basalis of Meynert (NBM) as seed and numerous regions of the paralimbic network. Smaller increases in FC were present between the central medial thalamic nucleus and retrosplenium seeds and the left caudate putamen and NBM. The results suggest that, during light anesthesia, PnO stimulation preferentially modulates basal forebrain-paralimbic networks. We speculate that this may be a reflection of disconnected awareness.