Nodularin from benthic freshwater periphyton and implications for trophic transfer
Nodularin from benthic freshwater periphyton and implications for trophic transfer
复制标题
DOI：
10.1016/j.toxicon.2017.10.023
复制
发表时间：
2017-12-15
期刊：
影响因子：
2.8
通讯作者：
Wertz, Tim
中科院分区：
文献类型：
--
作者：
Foss, Amanda J.;Butt, Jeffery;Wertz, Tim
2013年和2015年，宾夕法尼亚州环境保护部对美国宾夕法尼亚州萨斯奎哈纳、特拉华州和俄亥俄河流域的浮游栖息地进行了调查，以筛选藻类群落和小口鱼(Micropterus Dolomieu)中的微囊藻毒素/结节毒素(MCs/NODS)。采集附植生物(41个测点68个)、全鱼幼鱼(19个测点153个)和成鱼肝脏(16个测点115个)样品，用ADDA酶联免疫吸附试验(ELISA)进行筛查。用LC-MS/MS对MCS/NODS阳性的样品进行进一步分析，包括14种微囊藻毒素和NOD-R的变种以及MMPB技术。对47%的附生植物标本检测结果为阳性，其中NOD-R阳性(0.7-82.2 ng g(-1)D.W.)在20个样本中。在15份阳性的全鱼幼鱼样品中，有10份(0.8-16.7 ng g(-1)W.W.)被确认为NOD-R。8份肝脏标本中2份(1.7&2.8 ng g(-1)w.w.)。MMPB方法测定了围生动物(2.2-1269 ng g(-1)D.W.)、全鱼幼鱼(5.0-210 ng g(-1)D.W.)的总MC/NOD。成人肝脏(8.5-29.5 ng g(-1)D.W.)。这项工作表明，NOD-R存在于美国淡水底栖藻类中，这对监测和营养转移具有更广泛的意义。(C)2017爱思唯尔有限公司。保留所有权利。
In 2013 and 2015, the Pennsylvania Department of Environmental Protection conducted a survey of lotic habitats within the Susquehanna, Delaware, and Ohio River basins in Pennsylvania, USA, to screen for microcystins/nodularins (MCs/NODs) in algae communities and smallmouth bass (Micropterus dolomieu). Periphyton (68 from 41 sites), juvenile whole fish (153 from 19 sites) and adult fish liver (115 from 16 sites) samples were collected and screened using an Adda enzyme-linked immunosorbent assay (ELISA). Samples that were positive for MCs/NODs were further analyzed using LC-MS/MS, including 14 variants of microcystin and NOD-R and the MMPB technique. The ELISA was positive for 47% of the periphyton collections, with NOD-R confirmed (0.7-82.2 ng g(-1) d.w.) in 20 samples. NOD-R was confirmed in 10 of 15 positive juvenile whole fish samples (0.8-16.7 ng g(-1) w.w.) and in 2 of 8 liver samples (1.7 & 2.8 ng g(-1) w.w.). The MMPB method resulted in total MCs/NODs measured in periphyton (2.2-1269 ng g(-1) d.w.), juvenile whole fish (5.0-210 ng g(-1) d.w.) and adult livers (8.5-29.5 ng g(-1) d.w.). This work illustrates that NOD-R is present in freshwater benthic algae in the USA, which has broader implications for monitoring and trophic transfer. (C) 2017 Elsevier Ltd. All rights reserved.