Identification of Two Transcriptional Activators MabZIP4/5 in Controlling Aroma Biosynthetic Genes during Banana Ripening
Identification of Two Transcriptional Activators MabZIP4/5 in Controlling Aroma Biosynthetic Genes during Banana Ripening
复制标题
香蕉成熟过程中控制香气生物合成基因的两个转录激活因子 MabZIP4/5 的鉴定
DOI：
10.1021/acs.jafc.8b01435
复制
发表时间：
2018-06-20
影响因子：
6.1
通讯作者：
Kuang, Jian-Fei
中科院分区：
文献类型：
--
作者：
Guo, Yu-fan;Zhang, Yun-liang;Kuang, Jian-Fei
香蕉香气形成基因的转录调控仍然知之甚少。在这项工作中，我们发现，在香蕉成熟过程中，与22 ℃预存的香蕉相比，7 ℃预存的香蕉香气生物合成基因（包括MaOMT 1、MaMT 1、MaGT 1、MaBCAT 1、MaACY 1、MaAGT 1和BanAAT）以及两个bZIP基因（MabZIP 4和MabZIPS）的表达下调。此外，MabZIP 4和MabZIPS被证明能够激活这些芳香生物合成基因的转录。重要的是，MabZIP 4直接与BanAAT启动子结合，而MabZIPS通过G-box基序与MaMT 1、MaACY 1、MaAGT 1和BanAAT的启动子结合，暗示MabZIPs在控制香蕉香气生物合成中的多种功能意义。总的来说，这项工作为理解香蕉成熟过程中与香气形成相关的转录调控机制提供了新的见解。
The transcriptional regulation of aroma formation genes remains poorly understood in the banana. In this work, we found that the expressions of a subset of aroma biosynthetic genes including MaOMT1, MaMT1, MaGT1, MaBCAT1, MaACY1, MaAGT1, and BanAAT, as well as two bZIP genes, MabZIP4 and MabZIPS, were down-regulated when prestored at 7 degrees C compared to those prestored at 22 degrees C during the ripening process of banana. Furthermore, MabZIP4 and MabZIPS were shown to be able to activate the transcription of these aroma biosynthetic genes. Importantly, MabZIP4 directly binds to BanAAT promoter, while MabZIPS binds to the promoters of MaMT1, MaACY1, MaAGT1, and BanAAT via the G-box motif, implicating the diverse functional significances of MabZIPs in controlling aroma biosynthesis in banana. Overall, this work sheds new insights on the understanding of transcriptional regulatory mechanisms associated with aroma formation during banana ripening.