Mobilization of mesenchymal stem cells by stromal cell-derived factor-1 released from chitosan/tripolyphosphate/fucoidan nanoparticles
Mobilization of mesenchymal stem cells by stromal cell-derived factor-1 released from chitosan/tripolyphosphate/fucoidan nanoparticles
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DOI：
10.1016/j.actbio.2011.12.009
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发表时间：
2012-03-01
影响因子：
9.7
通讯作者：
Liu, Ting-Ju
中科院分区：
文献类型：
--
作者：
Huang, Yi-Cheng;Liu, Ting-Ju
基质细胞衍生因子-1（SDF-1）是一种重要的趋化因子，在间充质干细胞（MSC）的生物学和生理功能中起着关键作用。然而，SDF-1在组织再生中的使用受到两个缺点的限制，即其半衰期短和容易被酶降解。本研究探讨了SDF-1从壳聚糖基纳米颗粒（NP）中的释放，并评估了释放的SDF-1对MSC迁移的影响。在制备的基于壳聚糖的NP中，壳聚糖/三聚磷酸盐/岩藻依聚糖（CS/TPP/F）NP是用于SDF-1释放的最有效载体。CS/TPP/F NP是球形的并且有效地包封SDF-1。CS/TPP/F NP保护SDF-1免受蛋白水解和热处理，并控制其释放长达7天。SDF-1的释放浓度达到23 ng/ml。体外细胞实验表明，释放的SDF-1保留了其促有丝分裂活性，促进了MSC的迁移，并增强了PI 3 K的表达。生物相容性CS/TPP/F NP可有效作为SDF-1的递送和控制释放的载体，以在组织工程应用中动员干细胞。(c)2011 Acta Materialia Inc.由爱思唯尔有限公司出版。保留所有权利。
Stromal cell-derived factor 1 (SDF-1) is an important chemokine in stem cell mobilization, and plays a critical role in the biological and physiological functions of mesenchymal stem cells (MSC). However, the use of SDF-1 in tissue regeneration is limited by two drawbacks, which are its short half-life and ready degradation by enzymes. This study investigates the release of SDF-1 from chitosan-based nanoparticles (NP) and evaluates the effect of released SDF-1 on the migration of MSC. Among the prepared chitosan-based NP a chitosan/tripolyphosphate/fucoidan (CS/TPP/F) NP is the most effective carrier for SDF-1 release. CS/TPP/F NP are spherical and effectively encapsulate SDF-1. The CS/TPP/F NP protected SDF-1 against proteolysis and heat treatment and controlled its release for up to 7 days. The concentration of released SDF-1 reached 23 ng ml(-1). According to in vitro experiments on cells the released SDF-1 retained its mitogenic activity, promoted the migration of MSC and enhanced PI3K expression. Biocompatible CS/TPP/F NP may be effective as carriers for the delivery and controlled release of SDF-1 to mobilize stem cells in tissue engineering applications. (c) 2011 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.