Expression of a functional FcεRI on rat eosinophils and macrophages
Expression of a functional FcεRI on rat eosinophils and macrophages
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DOI：
10.4049/jimmunol.165.3.1266
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发表时间：
2000-08-01
影响因子：
4.4
通讯作者：
Capron, M
中科院分区：
文献类型：
--
作者：
Dombrowicz, D;Quatannens, B;Capron, M
除了在I型超敏反应中发挥重要作用外，IgE还参与抗寄生虫免疫。由于高亲和力IgE受体Fc epsilon RI在人和小鼠体内的细胞分布不同，这可能解释了两种血吸虫之间IgE效应功能的差异。在人类，嗜酸性粒细胞和巨噬细胞对曼氏血吸虫幼虫产生依赖于IgE的细胞毒作用，在嗜酸性粒细胞的情况下涉及Fc epsilon RI。在本研究中，我们研究了Fc epsilon RI在大鼠嗜酸性粒细胞和巨噬细胞中的表达和功能。通过流式细胞术、荧光显微镜和Western印迹分析，我们证明了在大鼠和人类中，功能正常的三聚体Fc epsilon RI在嗜酸性粒细胞和巨噬细胞上表达。我们还发现，这两种细胞类型都能诱导IgE介导的、Fc epsilon RI依赖的细胞对血吸虫的杀伤作用，这些结果为大鼠和小鼠在IgE介导的抗寄生虫免疫方面的差异提供了分子基础。
Besides its crucial role in type I hypersensitivity reactions, IgE is involved in anti-parasite immunity. This role has been clearly demonstrated in both human and rat schistosomiasis, but remains controversial in the mouse, Since the cellular distribution of the high affinity IgE receptor, Fc epsilon RI, differs in humans and mice, it might explain the differences in effector function of IgE between the two species, In humans, eosinophils and macrophages induce IgE-dependent cytotoxicity toward Schistosoma mansoni larvae, which involves Fc epsilon RI in the case of eosinophils. In the present study, we have investigated the expression and function of Fc epsilon RI in rat eosinophils and macrophages, We demonstrate, by flow cytometry, fluorescence microscopy, and Western blot analysis, that in rats, as in humans, a functional ay, trimeric Fc epsilon RI is expressed on eosinophils and macrophages. We also show that these two cell types can induce IgE-mediated, Fc epsilon RI dependent cellular cytotoxicity toward schistosomula, These results thus provide a molecular basis for the differences observed between rat and mouse regarding IgE-mediated anti-parasite immunity.