Recruitment of the Swi/Snf complex by Ste12-Tec1 promotes Flo8-Mss11-mediated activation of STA1 expression
Recruitment of the Swi/Snf complex by Ste12-Tec1 promotes Flo8-Mss11-mediated activation of STA1 expression
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DOI：
10.1128/mcb.24.21.9542-9556.2004
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发表时间：
2004-11-01
影响因子：
5.3
通讯作者：
Kang, HS
中科院分区：
文献类型：
--
作者：
Kim, TS;Kim, HY;Kang, HS
在糖化酵母中，编码胞外葡糖淀粉酶的STA 1基因的表达被特异性DNA结合激活剂Flo 8、Mss 11、Ste 12和Tec 1以及Swi/Snf染色质重塑复合物激活。在这里，我们表明Flo 8与Mss 11在物理和功能上相互作用。Flo 8和Mss 11协同结合STA 1启动子的UAS 1 -2中的反向重复序列TTTGC-n-GCAAA（n = 97）。此外，Flo 8和Mss 11通过与Ste 12和Tec 1相互作用而间接结合到STA 1启动子的UAS 2 -1，Ste 12和Tec 1结合到UAS 2 -1中的表达和侵袭反应元件（FRE）。此外，我们的研究结果表明，Ste 12，Tec 1，Flo 8和Mss 11激活剂和Swi/Snf复合物依次结合到STA 1启动子，如下：Ste 12和Tec 1首先结合到FRE，从而它们将Swi/Snf复合物募集到STA 1启动子。接下来，Swi/Snf复合物增强Flo 8和Mss 11与UAS 1 -2的结合。在最后一步中，Flo 8和Mss 11直接促进RNA聚合酶11与STA 1启动子的结合以激活STA 1表达。在没有葡萄糖的情况下，Flo 8和Tec 1的水平大大增加，而两个阻遏物，Nrg 1和Si 11的丰度减少，这表明转录调节因子的平衡可能是重要的确定激活或抑制STA 1表达。
In the yeast Saccharomyces diastaticus, expression of the STA1 gene, which encodes an extracellular glucoamylase, is activated by the specific DNA-binding activators Flo8, Mss11, Ste12, and Tec1 and the Swi/Snf chromatin-remodeling complex. Here we show that Flo8 interacts physically and functionally with Mss11. Flo8 and Mss11 bind cooperatively to the inverted repeat sequence TTTGC-n-GCAAA (n = 97) in UAS1-2 of the STA1 promoter. In addition, Flo8 and Mss11 bind indirectly to UAS2-1 of the STA1 promoter by interacting with Ste12 and Tec1, which bind to the filamentation and invasion response element (FRE) in UAS2-1. Furthermore, our findings indicate that the Ste12, Tec1, Flo8, and Mss11 activators and the Swi/Snf complex bind sequentially to the STA1 promoter, as follows: Ste12 and Tec1 bind first to the FRE, whereby they recruit the Swi/Snf complex to the STA1 promoter. Next, the Swi/Snf complex enhances Flo8 and Mss11 binding to UAS1-2. In the final step, Flo8 and Mss11 directly promote association of RNA polymerase 11 with the STA1 promoter to activate STA1 expression. In the absence of glucose, the levels of Flo8 and Tec1 are greatly increased, whereas the abundances of two repressors, Nrg1 and Si11, are reduced, suggesting that the balance of transcriptional regulators may be important for determining activation or repression of STA1 expression.