In vivo non-specific binding of λ CI and Cro repressors is significant
In vivo non-specific binding of λ CI and Cro repressors is significant
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DOI：
10.1016/s0014-5793(04)00249-2
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发表时间：
2004-04-09
期刊：
影响因子：
3.5
通讯作者：
Metzler, R
中科院分区：
文献类型：
--
作者：
Bakk, A;Metzler, R
我们提出了一个包含 lambda 非特异性结合的热力学模型。噬菌体调节蛋白 CI 和 Cro。通过将模型与两种启动子 P-RM 和 P-R 活性与浓度的体内实验数据进行拟合，我们估计 CI 的非特异性结合自由能为 -4.1 +/- 0.9 kcal/mol，Cro 的非特异性结合自由能为 -4.2 +/- 0.8 kcal/mol。对于浓度 > 100 nM 的 CI 或 Cro，我们发现 > 50% 的这些蛋白质是非特异性结合的。特别是，在溶原（类似于每个细胞 250 CI 单体当量）中，近 90% 的 CI 是非特异性结合的。 (C) 2004 年欧洲生化学会联合会。由 Elsevier B.V. 出版。保留所有权利。
We propose a thermodynamic model that includes the non-specific binding of the lambda. phage regulatory proteins CI and Cro. By fitting the model to experimental in vivo data on activities of the two promoters P-RM and P-R versus concentration, we estimate the free energy upon non-specific binding to be -4.1 +/- 0.9 kcal/mol for CI and -4.2 +/- 0.8 kcal/mol for Cro. For concentrations > 100 nM of CI or Cro, we find that > 50% of these proteins are non-specifically bound. In particular, in a lysogen (similar to250 CI monomeric equivalents per cell) nearly 90% of CI is non-specifically bound. (C) 2004 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.