Endothelial colony-forming cells: Biological and functional abnormalities in patients with recurrent, unprovoked venous thromboembolic disease
Endothelial colony-forming cells: Biological and functional abnormalities in patients with recurrent, unprovoked venous thromboembolic disease
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DOI：
10.1016/j.thromres.2015.11.005
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发表时间：
2016-01-01
影响因子：
7.5
通讯作者：
Majluf-Cruz, Abraham
中科院分区：
文献类型：
--
作者：
Antonio Alvarado-Moreno, Jose;Hernandez-Lopez, Rubicel;Majluf-Cruz, Abraham
简介：内皮细胞（EC）是血液凝固系统的重要组成部分，因为它维持血液。因为在静脉血栓栓塞性疾病（VTD）的患者中，有时没有发现嗜血栓性的条件，我们调查，如果内皮细胞集落形成细胞（ECFCs）从这些patients有生物学和功能abnormality.Patients和方法：人单核细胞（MNCs）从外周血中获得与VTD和对照患者，以获得ECFCs。这些细胞的免疫表型和电子显微镜特征和他们的能力，形成毛细血管样结构，并产生促炎和促血管生成的细胞因子和活性氧（ROS）测定结果：ECFCs出现在7和21天的VTD患者和对照组的文化，分别。患者ECFC增加8倍，并提前1周出现。各组间ECFC集落大小无差异，ECFC出现的数量和时间不同。两组ECFC衍生的EC（ECFC-EC）均表达CD 31、CD 34、CD 146和CD-309，但均不表达CD 45、CD 14或CD 90。目的CD 34在患者ECFC-ECs中高表达。在两组中，ECFC-EC显示出相似的形成毛细血管样结构的能力，但来自患者的ECFC-EC在线粒体膜上有显著的异常。我们发现来自患者的ECFC-EC中ROS产生显著增加。VTD患者和controls.Conclusions之间的细胞因子谱有显着差异：我们发现一个功能失调的状态ECFC从VTD患者类似功能失调的EC的一些特点。这些发现可能有助于了解VTD的一些病理生理方面。(C)2015爱思唯尔有限公司版权所有。
Introduction: Endothelial cells (ECs) are an important component of the blood coagulation system because it maintains blood fluid. Because in patients with venous thromboembolic disease (VTD) a thrombophilic condition is not found sometimes, we investigated if endothelial colony-forming cells (ECFCs) from these patients have biological and functional abnormalities.Patients and methods: Human mononuclear cells (MNCs) were obtained from peripheral blood from patients with VTD and controls to obtain ECFCs. These cells were assayed for their immunophenotype and electron microscopy characteristics and their ability to form capillary-like structures and to produce pro-inflammatory and pro-angiogenic cytokines and reactive oxygen species (ROS).Results: ECFCs appeared at 7 and 21 days of culture in VTD patients and controls, respectively. ECFCs increased 8-fold in patients and emerged 1 week earlier. No differences in the size of the colonies of ECFCs were found. Numbers and time of appearance of ECFCs was different between groups. ECFC-derived ECs (ECFC-ECs) of both groups expressed CD31, CD34, CD146, and CD-309 but none expressed CD45, CD14, or CD90. Interest CD34 was highly expressed in ECFC-ECs from patients. In both groups, ECFC-ECs showed similar capacity to form capillary-like structures but ECFC-ECs from patients had significant abnormalities in the mitochondrial membrane. We found a significant increase in ROS production in ECFC-ECs from patients. There were significant differences in cytokine profiles between VTD patients and controls.Conclusions: We found a dysfunctional state in ECFC from VTD patients resembling some characteristics of dysfunctional ECs. These findings may help to understand some pathophysiological aspects of VTD. (C) 2015 Elsevier Ltd. All rights reserved.