The lipid lowering and antioxidative stress potential of polysaccharide from Auricularia auricula prepared by enzymatic method
The lipid lowering and antioxidative stress potential of polysaccharide from Auricularia auricula prepared by enzymatic method
复制标题
酶法制备黑木耳多糖的降脂及抗氧化应激潜力
DOI：
10.1016/j.ijbiomac.2021.07.138
复制
发表时间：
2021-08-01
影响因子：
8.2
通讯作者：
Zhang, Yongjun
中科院分区：
文献类型：
--
作者：
Xiao, Bin;Chen, Shuang;Zhang, Yongjun
采用中性蛋白酶法提取黑木耳多糖，并采用响应面分析法对提取工艺进行优化。采用分步乙醇沉淀法对AAPs进行分级，得到回收率高、清除自由基能力强的AAPs级分，并以秀丽隐杆线虫为模型生物，研究其抗氧化和降血脂作用。在50 ℃、液料比75 mL/g、pH 9.0条件下，AAP的提取率和ABTS（+）清除率分别达到14.90%和86.0%。经15%乙醇提取得到的AAP 3是由甘露糖、葡萄糖、葡萄糖醛酸、木糖、半乳糖和氨基葡萄糖组成的杂多糖。AAP 3能显著延长C. 0.25mg/mL的超氧化物歧化酶（SOD）、过氧化氢酶（CAT）活性显著提高（p <0.05）。qRT-PCR结果显示，0.25 mg/mL AAP 3可上调daf-16和skn-1的mRNA表达水平（> 1.6倍）。AAP 3能显著降低C.线虫（p <0.05）。这些研究表明，A.中性蛋白酶法制备的黑木耳多糖对细胞内自由基产生剂引起的细胞损伤具有显著的保护作用。
An efficient extraction method of Auricularia auricula polysaccharides (AAPs) by neutral protease was developed and optimized by response surface methodology. AAPs were graded by stepwise ethanol precipitation, the fraction with high recovery rate and strong radical scavenging rate were obtained, then its antioxidant and lipid lowering effect were studied using Caenorhabditis elegans as model organism. The extract yield and ABTS(+) scavenging rates of AAPs could reach 14.90% and 86.0% at 50 degrees C, 75 mL/g of liquid-to-material ratio and pH 9.0. AAP3 obtained by 15% ethanol was a heteropolysaccharide comprised of mannose, glucose, glucuronic acid, xylose, galactose and glucosamine. AAP3 could significantly prolong the lifespan of C. elegans and enhance the activity of antioxidant enzymes including superoxide dismutase (SOD), catalases (CAT) at 0.25 mg/mL (p < 0.05). The qRT-PCR results showed that AAP3 could up regulate mRNA expression levels of daf-16 and skn-1 (>1.6 fold) at 0.25 mg/mL. Besides, AAP3 could significantly reduce the level of body fat and triglyceride in C. elegans (p < 0.05). These studies demonstrated that A. auricula polysaccharides prepared by neutral protease had a prominent protective effect to the damage induced by the intracellular free radical generating agents.