Suppressive effect of Astragalus membranaceus Bunge on chemical hepatocarcinogenesis in rats
Suppressive effect of Astragalus membranaceus Bunge on chemical hepatocarcinogenesis in rats
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DOI：
10.1007/s00280-002-0532-5
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发表时间：
2003-01-01
影响因子：
3
通讯作者：
Shen, H
中科院分区：
文献类型：
--
作者：
Cui, R;He, JC;Shen, H
黄芪（Astragalus membranaceus，AM）是传统中医药治疗肝病的常用药。实验证据表明它具有抗肿瘤潜力。本研究以胎盘型谷胱甘肽S-转移酶（GST-P）为标志物，观察黄芪对二乙基亚硝胺（DEN）、2/3肝部分切除术和2-乙酰氨基芴（2-AAF）诱发大鼠肝癌（DEN-PH-AAF）的影响。首先，将大鼠腹膜内（i. p.）用DEN（200 mg/kg生理盐水）诱发大鼠肝癌，2周后行2/3部分肝切除术，第3 ~ 8周喂以含0.02%AAF的基础饲料诱发大鼠肝癌。从第3周至第8周（治疗组），通过灌胃给予AM（90 mg/kg或180 mg/kg体重）。AM能明显抑制DEN-PH-AAF诱导的肝癌细胞GST-P阳性灶的形成，降低DEN-PH-AAF诱导的肝癌细胞GST-P蛋白和mRNA的表达，提示AM能有效延缓DEN-PH-AAF诱导的肝癌发生。
Astragalus membranaceus (AM) has been widely used for treating liver diseases in traditional Chinese medicine. Experimental evidence indicates that it has antitumor potential. In this study, the effect of AM on hepatocarcinogenesis induced by diethylnitrosamine (DEN), two-thirds partial hepatectomy, and 2-acetylaminofluorene (2-AAF) (DEN-PH-AAF) was evaluated using glutathione S-transferase placenta form (GST-P) as marker. First, rats were injected intraperitoneally (i.p.) with DEN (200 mg/kg in saline), a two-thirds partial hepatectomy was carried out 2 weeks later, and the rats were then placed on a basal diet containing 0.02% AAF from week 3 to week 8 to induce hepatocarcinogenesis. The rats were given AM (90 mg/kg or 180 mg/kg body weight) by gavage from week 3 to week 8 (treatment groups). The formation of GST-P-positive foci and the expression of GST-P protein and mRNA caused by DEN-PH-AAF were reduced in the treatment groups, which clearly suggests that AM is effective in delaying DEN-PH-AAF-induced hepatocarcinogenesis.