Laminar distribution of neuronal membrane properties in neocortex of normal and reeler mouse.
Laminar distribution of neuronal membrane properties in neocortex of normal and reeler mouse.
复制标题
正常小鼠和 reeler 小鼠新皮质神经元膜特性的层状分布。
DOI：
10.1152/jn.1991.66.6.2034
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发表时间：
1991
影响因子：
2.5
通讯作者：
Connors,BW
中科院分区：
文献类型：
--
作者：
Silva,LR;Gutnick,MJ;Connors,BW
1. Reeler是一种常染色体隐性突变的小鼠，在发育过程中改变神经元迁移，产生新皮质中的层的一般倒置。我们在体外记录正常和卷轴新皮层的切片，以研究神经元的位置和形状对膜特性和突触反应的影响。2.内在的放电模式，动作电位的形状，静息膜电位，输入电阻，和诱发兴奋性突触后电位（EPSP）和抑制性突触后电位（IPSP）没有不同reelers和控制数据分组时。3.内在的放电模式的深度分布是颠倒的卷轴：内在的爆裂（IB）神经元被发现只在第5层在正常小鼠，但他们被发现专门在颗粒层的卷轴皮质。4.在卷轴的突触反应的空间分布也被倒置：非常突出的IPSPs的特点是在正常小鼠的上层神经元，但在卷轴类似的抑制反应，主要是在深颗粒下层观察。5.染料注射在reeler锥体神经元显示非典型的形态，包括扭曲的顶端树突和细胞倒置。6.这些数据意味着，皮质神经元发育出与其功能相适应的膜和突触特性，尽管它们是畸形的和错位的。
1. Reeler is an autosomal recessive mutation of mice that alters neuronal migration during development, yielding a general inversion of the laminae in the neocortex. We recorded in vitro from slices of normal and reeler neocortex to study the influence of neuron position and shape on membrane properties and synaptic responses. 2. The intrinsic firing patterns, action-potential shapes, resting membrane potentials, input resistances, and evoked excitatory postsynaptic potentials (EPSPs) and inhibitory postsynaptic potentials (IPSPs) did not differ between reelers and controls when data were grouped. 3. The depth distribution of intrinsic firing patterns was inverted in the reeler: intrinsically bursting (IB) neurons were found only in layer 5 in the normal mouse, but they were found exclusively in supragranular layers of the reeler cortex. 4. The spatial distribution of synaptic responses in the reeler was also inverted: very prominent IPSPs were characteristic of upper layer neurons in the normal mouse, but in the reeler similar inhibitory responses were observed predominantly in deep infragranular layers. 5. Dye injections in reeler pyramidal neurons revealed atypical morphologies, including distorted apical dendrites and cell inversion. 6. The data imply that cortical neurons develop the membrane and synaptic properties appropriate to their function, despite being malformed and mispositioned.