An amperometric glucose biosensor based on the immobilization of glucose oxidase on the ZnO nanotubes
An amperometric glucose biosensor based on the immobilization of glucose oxidase on the ZnO nanotubes
复制标题
基于氧化锌纳米管固定化葡萄糖氧化酶的电流型葡萄糖生物传感器
DOI：
10.1016/j.snb.2009.01.002
复制
发表时间：
2009-04-24
影响因子：
8.4
通讯作者：
Wang, Xiaoping
中科院分区：
文献类型：
--
作者：
Kong, Tao;Chen, Yang;Wang, Xiaoping
采用交联法将葡萄糖氧化酶固定在氧化锌纳米管阵列上，制备了葡萄糖生物传感器。ZnO纳米管阵列是通过电化学沉积在Au表面的ZnO纳米棒的化学蚀刻合成的。用场发射扫描电镜（FESEM）、高分辨透射电镜（HRTEM）和X射线衍射（XRD）对ZnO纳米管的形貌和结构进行了表征。傅立叶变换红外光谱表明，葡萄糖氧化酶固定在氧化锌纳米管保持其天然构象。该传感器具有较宽的线性范围内检测葡萄糖从50 μ M到12 mM（相关系数为0.998）与3s的响应时间。该传感器的灵敏度为21.7 μ A/mM cm（2）。实验检测限为1 μ M（S/N = 3），表观米氏常数为19 mM，并对该传感器的抗干扰能力和长期稳定性进行了评价。与基于纳米棒和平面结构的生物传感器相比，该传感器具有更大的线性范围和灵敏度。这些结果表明ZnO纳米管可以为生物传感器的设计和其他生物应用提供一种有前途的材料。(C)2009 Elsevier B. V.保留所有权利。
A glucose biosensor is fabricated with immobilization of glucose oxidase onto ZnO nanotube arrays by cross-linking method. The ZnO nanotube arrays are synthesized by chemical etching of ZnO nanorods that are electrochemically deposited on the Au surface. Morphology and structure of ZnO nanotubes are characterized by FESEM, HRTEM and XRD. Fourier-trans form infrared spectroscopy reveals that the glucose oxidase immobilized on the ZnO nanotubes retains its native conformation. The biosensor has a wide linear range for the detection of glucose from 50 mu M to 12 mM (a correlation coefficient of 0.998) with 3s response time. The sensitivity of the biosensor is found to be 21.7 mu A/mM cm(2). Moreover, its experimental detection limit is I mu M (S/N = 3) and the apparent Michaelis-Men ten constant is calculated to be 19 mM. The anti-interference ability and long-term stability of the biosensor are also assessed. Compared with the biosensors based on the nanorod and flat structure, the proposed biosensor shows expanded linear range and sensitivity. All these results demonstrate that ZnO nanotube can provide a promising material for the biosensor designs and other biological applications. (C) 2009 Elsevier B.V. All rights reserved.