ITS-PCR-RFLP method for distinguishing commercial cultivars of edible mushroom, Flammulina velutipes
ITS-PCR-RFLP method for distinguishing commercial cultivars of edible mushroom, Flammulina velutipes
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DOI：
10.1111/j.1365-2621.2002.tb08763.x
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发表时间：
2002-09-01
影响因子：
3.9
通讯作者：
Morinaga, T
中科院分区：
文献类型：
--
作者：
Palapala, VA;Aimi, T;Morinaga, T
采用核苷酸序列测定和聚合酶链反应（PCR）-限制性片段长度多态性（RFLP）技术，对7个典型金针菇菌株的核糖体RNA基因（rDNA）内转录间隔区（ITS）和5.8SrRNA编码区进行了分析。这些核苷酸序列揭示了菌株特异性缺失、插入和取代的存在。此外，使用限制性内切酶DraI、FokI、Hae II、Mbo II和NlarV产生的RFLP模式能够鉴定特定的Pammulina菌株。因此，ITS区的PCR-RFLP分析似乎是一个有用的工具，用于鉴定Flaninudina菌株。
Nucleotide sequence and polymerase chain reaction (PCR) - restriction fragment length polymorphism (RFLP) analysis of the ribosomal RNA gene (rDNA) regions containing the internal transcribed spacers (ITSs) and 5.8S rRNA coding sequence was used to differentiate between 7 typical Flammulina strains. These nucleotide seqeuences revealed the presence of strain-specific deletions, insertions, and substitutions. Moreover, RFLP patterns produced using restriction endonucleases DraI, FokI, HaeII, MboII, and NlarV, enabled Identification of specific Pammulina strains. Thus, PCR-RFLP analysis of the ITS regions appears to be a useful tool for the identification of Flaninudina strains.