Two distinct groups within the Bacillus subtilis group display significantly different spore heat resistance properties
Two distinct groups within the Bacillus subtilis group display significantly different spore heat resistance properties
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DOI：
10.1016/j.fm.2014.04.009
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发表时间：
2015-02-01
影响因子：
5.3
通讯作者：
Wells-Bennik, Marjon H. J.
中科院分区：
文献类型：
--
作者：
Berendsen, Erwin M.;Zwietering, Marcel H.;Wells-Bennik, Marjon H. J.
热处理后细菌孢子的存活以及随后在食品中的萌发和生长可导致食品的腐败和经济损失。预测导致充分灭活的时间-温度条件需要获得相关模型菌株的详细孢子热灭活动力学。在这项研究中，热灭活动力学属于枯草芽孢杆菌组的14个菌株的孢子进行了详细的测定，使用批量加热毛细管和连续流动加热的微型加热器。使用对数线性模型拟合灭活数据。基于孢子耐热性数据，在B内有两个不同的组（p < 0.001）。subtilis群。一组菌株的孢子的平均D-120（摄氏度）为0.33秒，而另一组的孢子显示出明显更高的耐热性，平均D-120摄氏度为45.7秒。当比较使用分批和连续流动加热获得的孢子灭活数据时，z值显著不同，因此从一个系统外推到另一个系统是不合理的。该研究清楚地表明，来自B中不同菌株的孢子的耐热性。subtilis组可以变化很大。菌株可以分为两组，不同的孢子热灭活动力学适用于这两组。(C)2014爱思唯尔有限公司版权所有。
The survival of bacterial spores after heat treatment and the subsequent germination and outgrowth in a food product can lead to spoilage of the food product and economical losses. Prediction of time-temperature conditions that lead to sufficient inactivation requires access to detailed spore thermal inactivation kinetics of relevant model strains. In this study, the thermal inactivation kinetics of spores of fourteen strains belonging to the Bacillus subtilis group were determined in detail, using both batch heating in capillary tubes and continuous flow heating in a micro heater. The inactivation data were fitted using a log linear model. Based on the spore heat resistance data, two distinct groups (p < 0.001) within the B. subtilis group could be identified. One group of strains had spores with an average D-120 (degrees C) of 0.33 s, while the spores of the other group displayed significantly higher heat resistances, with an average D-120 degrees C of 45.7 s. When comparing spore inactivation data obtained using batch-and continuous flow heating, the z-values were significantly different, hence extrapolation from one system to the other was not justified. This study clearly shows that heat resistances of spores from different strains in the B. subtilis group can vary greatly. Strains can be separated into two groups, to which different spore heat inactivation kinetics apply. (C) 2014 Elsevier Ltd. All rights reserved.