Differential expression of three types of nitric oxide synthase in both infarcted and non-infarcted left ventricles after myocardial infarction in the rat
Differential expression of three types of nitric oxide synthase in both infarcted and non-infarcted left ventricles after myocardial infarction in the rat
复制标题
DOI：
10.1016/s0167-5273(00)00394-6
复制
发表时间：
2000-11-01
影响因子：
3.5
通讯作者：
Sasayama, S
中科院分区：
文献类型：
--
作者：
Takimoto, Y;Aoyama, T;Sasayama, S
在本报告中，我们研究了大鼠心肌梗死后左心室三种类型一氧化氮合酶（NOS）的差异表达。结扎冠状动脉后1、3、7、14、28和56天(每组n＝6-12)，从梗塞和非梗塞组织获取组织样品。通过半定量逆转录聚合酶链反应和蛋白质印迹依次测定神经元（n）NOS、内皮（e）NOS和诱导型（i）NOS的mRNA和蛋白水平。超声心动图证实进行性左心室扩张和缩短分数逐渐减少。与假手术大鼠相比，梗死后1、3、7天nNOS表达水平显着升高（P
In the present report we investigated the differential expression of three types of nitric oxide synthase (NOS) in the left ventricle after myocardial infarction in rats. One, 3, 7, 14, 28 and 56 days (n=6-12 for each group) after ligation of a coronary artery, tissue samples were obtained from infarcted and non-infarcted tissues. The mRNA and protein levels of neuronal (n) NOS, endothelial (e) NOS and inducible (i) NOS were sequentially determined by semi-quantitative reverse transcription-polymerase chain reaction and Western blotting. Progressive left ventricular dilatation and gradual reduction in fractional shortening were confirmed by echocardiography. The expression levels of nNOS were significantly increased 1, 3 and 7 days post-infarct compared to those of sham-operated rats in both the infarcted (P