Cloning, characterization, and sequence of a porcine cDNA encoding a secreted neuronal and endocrine protein.
Cloning, characterization, and sequence of a porcine cDNA encoding a secreted neuronal and endocrine protein.
复制标题
编码分泌性神经元和内分泌蛋白的猪 cDNA 的克隆、表征和序列。
DOI：
10.1089/dna.1988.7.713
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发表时间：
1988
期刊：
影响因子：
--
通讯作者：
Dixon,JE
中科院分区：
文献类型：
--
作者：
Brayton,KA;Aimi,J;Qiu,H;Yazdanparast,R;Ghatei,MA;Polak,JM;Bloom,SR;Dixon,JE
该报告描述了 cDNA 的克隆、序列和表征，该 cDNA 编码在大脑和内分泌组织（包括垂体、肾上腺髓质和卵巢）中合成的蛋白质。推导的 23 kD 蛋白质的 207 个氨基酸序列包含疏水信号肽，表明它是分泌的。利用 cDNA 克隆进行 Northern 印迹分析，鉴定出猪脑、肾上腺髓质、垂体和卵巢以及人类内分泌肿瘤中约 1400 个核苷酸的单个 RNA。在一种人类胰腺肿瘤中观察到非常高水平的 RNA。 Southern 印迹分析表明，与猪 cDNA 同源的序列存在于人类、牛、大鼠和鲑鱼 DNA 中，表明这些基因在进化过程中高度保守。
This report describes the cloning, sequence, and characterization of a cDNA which encodes a protein synthesized in the brain and endocrine tissues, including pituitary, adrenal medulla, and ovary. The deduced 207-amino-acid sequence of the 23-kD protein contains a hydrophobic signal peptide suggesting that it is secreted. Northern blot analysis utilizing the cDNA clone identifies a single RNA of approximately 1400 nucleotides in porcine brain, adrenal medulla, pituitary, and ovary, as well as in human endocrine tumors. Very high levels of RNA were observed in one human pancreatic tumor. Southern blot analysis suggests that sequences homologous to the porcine cDNA are present in human, cow, rat, and salmon DNA, indicating that the gene(s) have been highly conserved during evolution.