Characterization of a novel circo-like virus in Aedes vexans mosquitoes from Germany: evidence for a new genus within the family Circoviridae
Characterization of a novel circo-like virus in Aedes vexans mosquitoes from Germany: evidence for a new genus within the family Circoviridae
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DOI：
10.1099/vir.0.000036
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发表时间：
2015-04-01
影响因子：
3.8
通讯作者：
Cadar, Daniel
中科院分区：
文献类型：
--
作者：
Garigliany, Mutien-Marie;Boerstler, Jessica;Cadar, Daniel
近几十年来，元基因组学研究扩大了圆环病毒科中新描述的、通常是未分类的病毒的数量。利用广谱环状病毒和环状病毒PCRS，我们在德国的斑纹伊蚊体内鉴定了一种新的环状病毒样病毒，它的主要开放阅读框与以前描述的环状病毒和环状病毒的氨基酸同源性很低。系统发育和遗传距离分析表明，这一新的病毒物种与以前描述的蚊虫和蝙蝠粪便衍生的Circo样病毒一起，属于圆环病毒科的一个不同的属，暂定为KrikoVirus。我们进一步证明，可能属于克里科病毒属的所有病毒都共享一个在圆环病毒科中独一无二的基因组组织。寄主范围、组织嗜性和传播途径有待进一步研究(S)。这份报告增加了关于圆环病毒科成员遗传多样性和进化的当前知识。
Over recent decades, metagenomic studies have expanded the number of newly described, often unclassified, viruses within the family Circoviridae. Using broad-spectrum circovirus and cyclovirus PCRs, we characterized a novel circo-like virus in Aedes vexans mosquitoes from Germany whose main putative ORFs shared very low amino acid identity with those of previously characterized circoviruses and cycloviruses. Phylogenetic and genetic distance analysis revealed that this new virus species defined, together with previously described mosquito- and bat faeces-derived circo-like viruses, a different genus, tentatively called Krikovirus, within the family Circoviridae. We further demonstrated that viruses of the putative genus Krikovirus all shared a genomic organization that was unique among the family Circoviridae. Further investigations are needed to determine the host range, tissue tropism and transmission route(s). This report increases the current knowledge of the genetic diversity and evolution of the members of the family Circoviridae.