Crystallization and preliminary X-ray analysis of D-monellin
Crystallization and preliminary X-ray analysis of D-monellin
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DOI：
10.1107/s0907444996014989
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发表时间：
1997-05-01
期刊：
影响因子：
--
通讯作者：
Kim, SH
中科院分区：
文献类型：
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作者：
Hung, LW;Kohmura, M;Kim, SH
D-Monellin是一种化学合成的蛋白质，由所有d -氨基酸组成。它的氨基酸序列与L-monellin相同，L-monellin是一种具有强甜味的天然蛋白质。得到了D-monellin的两种结晶形式。两种晶体都是在与天然L-monellin结晶相似的条件下生长的。晶体形式I具有与天然L-monellin相似但不相同的细胞参数，衍射分辨率为2.7埃。晶体形式II是非常不同的，衍射到1.7埃分辨率使用同步辐射。
D-Monellin is a chemically synthesized protein composed of all D-amino acids. It has an amino-acid sequence identical to L-monellin, a natural protein with potent sweetness. Two crystal forms of D-monellin were obtained. Both crystals were grown under conditions similiar to those used to crystallize natural L-monellin. Crystal form I has similar, but not identical, cell parameters to natural L-monellin and diffracts to 2.7 Angstrom resolution. Crystal form II is very different and diffracts to 1.7 Angstrom resolution using synchrotron radiation.