Frequencies of X-ray induced pericentric inversions and centric rings in human blood lymphocytes detected by FISH using chromosome arm specific DNA libraries
Frequencies of X-ray induced pericentric inversions and centric rings in human blood lymphocytes detected by FISH using chromosome arm specific DNA libraries
复制标题
DOI：
10.1016/s0027-5107(96)00209-6
复制
发表时间：
1996-11-11
影响因子：
2.3
通讯作者：
Balajee, AS
中科院分区：
文献类型：
--
作者：
Natarajan, AT;Boei, JJWA;Balajee, AS
在X射线照射（2.5戈伊）的人淋巴细胞中，染色体内交换（臂间倒位和中心环）和染色体间交换（双着丝粒和易位）的频率已被估计。为了检测这些事件，我们采用FISH（荧光原位杂交）技术和染色体#1和#3的臂特异性涂染探针。中心环和臂间倒位的比值约为1。对于内部变化与相互变化，比率（F）在6和9之间。基于涉及染色体#1和#3的颜色连接的总数，发现相同染色体的臂之间的交换比基于染色体的DNA含量和总基因组中畸变的随机诱导计算的染色体间交换多约8.7倍。间期核中的染色体组织似乎促进了X射线照射后更多的内部变化而不是内部变化的形成，这很可能是由于染色体的两个臂非常接近。
Frequencies of intra-chromosomal exchanges (pericentric inversions and centric rings) and inter-chromosomal exchanges (dicentrics and translocations) in X-irradiated (2.5 Gy) human lymphocytes have been estimated. To detect these events we employed FISH (fluorescence in situ hybridization) technique and arm specific painting probes for chromosomes #1 and #3. The ratio between centric rings and pericentric inversions was found to be about 1. For intra-changes to inter-changes, the ratio (F) was between 6 and 9. Based on the total number of colour junctions involving chromosomes #1 and #3 it was found that exchanges between the arms of the same chromosome occur about 8.7 times more than inter-chromosomal exchanges calculated on the basis of the DNA content of the chromosomes and random induction of aberrations in the total genome. Chromosomal organization in interphase nucleus appears to promote the formation of more intra-changes than inter-changes following X-irradiation, most probably due to close proximity of the two arms of a chromosome.