Large scale preparation of wheat germ agglutinin.
Large scale preparation of wheat germ agglutinin.
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小麦胚芽凝集素的大规模制备。
DOI：
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发表时间：
1975
期刊：
影响因子：
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通讯作者：
E. Bassett
中科院分区：
文献类型：
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作者：
E. Bassett
用 0.5 M 甲酸提取麦芽，并在亲和色谱前后用蒸馏水透析除去变性或不溶于水的 WGA，然后在部分酸水解的几丁质柱上通过亲和色谱分离出高活性形式的麦芽凝集素 (WGA)。通过凝胶过滤、圆盘电泳和化学分析发现纯化的制剂是均质的。它很容易与人血清和尿液中的 WGA 受体发生反应，在琼脂凝胶双扩散和电泳上产生清晰的条带。当与琼脂糖凝胶化学偶联时，WGA 与红细胞、血清、尿液和其他生物液体中的 WGA 受体反应非常强烈。 Sepharose-WGA 已被证明可以长期保持稳定。
A highly active form of wheat germ agglutinin (WGA) was isolated by affinity chromatography on a partially acid hydrolyzed chitin column after extraction of the wheat germ with 0.5 M formic acid and removal of the denatured or water insoluble WGA by dialyzing against distilled water before and after affinity chromatography. The purified preparation was found to be homogeneous by gel filtration, disc electrophoresis, and chemical analysis. It reacted readily with WGA receptors in human serum and urine, giving well-defined bands on agar gel double diffusion and electrophoresis. When chemically coupled to Sepharose the WGA was very reactive with red blood cells, WGA receptors in serum, urine and other biological fluids. The Sepharose-WGA has proven to be stable over a long period of time.