THE ESCHERICHIA-COLI HEAT-SHOCK GENE HTPY - MUTATIONAL ANALYSIS, CLONING, SEQUENCING, AND TRANSCRIPTIONAL REGULATION
THE ESCHERICHIA-COLI HEAT-SHOCK GENE HTPY - MUTATIONAL ANALYSIS, CLONING, SEQUENCING, AND TRANSCRIPTIONAL REGULATION
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DOI：
10.1128/jb.175.9.2613-2624.1993
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发表时间：
1993-05-01
影响因子：
3.2
通讯作者：
RAINA, S
中科院分区：
文献类型：
--
作者：
MISSIAKAS, D;GEORGOPOULOS, C;RAINA, S
我们已经确定了一个新的热休克基因，指定htpY，位于上游700 bp的dnaK dnaJ操纵子。我们克隆了它，并表明它是顺时针转录维斯大肠杆菌遗传图谱，在相同的方向作为dnaK dnaJ操纵子。 htpY基因编码21，193-Da多肽。启动子定位实验和北方（RNA）分析表明htpY基因属于经典的热休克基因家族，因为其主要启动子的转录受rpoH基因prodUCt（sigma 32）的正控制，并且类似于典型的Esigma 32转录的共有启动子序列。phtpY-lacZ启动子融合体的构建和分析加强了这一结论。尽管htpY无效细菌是可行的，但各种Esigma 32热休克启动子的表达显著降低，表明HtpY在调节热休克反应中起重要作用。与这种解释一致，HtpY蛋白的过量产生导致E.杆菌
We have identified a new heat shock gene, designated htpY, located 700 bp upstream of the dnaK dnaJ operon. We cloned it and showed that it is transcribed clockwise vis-a-vis the Escherichia coli genetic map, in the same direction as the dnaK dnaJ operon. The htpY gene encodes a 21,193-Da polypeptide. Promoter mapping experiments and Northern (RNA) analysis showed that the htpY gene belongs to the classical heat shock gene family, because the transcription from its major promoter is under the positive control of the rpoH gene prodUCt (sigma32) and resembles canonical Esigma32-transcribed consensus promoter sequences. This conclusion has been strengthened by the construction and analysis of a phtpY-lacZ promoter fusion. Despite the fact that htpY null bacteria are viable, the expression of various Esigma32 heat shock promoters is significantly decreased, suggesting that HtpY plays an important role in the regulation of the heat shock response. Consistent with this interpretation, overproduction of the HtpY protein results in a generalized increase of the heat shock response in E. coli.