Melatonin promotes Cashmere goat (Capra hircus) secondary hair follicle growth: a view from integrated analysis of long non-coding and coding RNAs
Melatonin promotes Cashmere goat (Capra hircus) secondary hair follicle growth: a view from integrated analysis of long non-coding and coding RNAs
复制标题
褪黑激素促进绒山羊 (Capra hircus) 次生毛囊生长：来自长非编码和编码 RNA 综合分析的观点
DOI：
10.1080/15384101.2018.1471318
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发表时间：
2018-01-01
期刊：
影响因子：
4.3
通讯作者：
Wang, Xin
中科院分区：
文献类型：
--
作者：
Ge, Wei;Wang, Shan-He;Wang, Xin
摘要几十年来，褪黑激素在促进羊绒山羊羊毛产量方面的作用已得到证实，但人们对褪黑激素介导的毛囊生长仍然缺乏了解。最近的研究表明，长链非编码RNA（lncRNA）在基因组中广泛转录，并在调节生物过程中发挥普遍作用。然而，lncRNA在调节褪黑激素介导的毛囊生长中的作用仍不清楚。本研究建立了绒山羊毛囊体外培养体系，并证明500 ng/L褪黑激素对毛囊纤维的生长有促进作用。基于长间隔RNA测序，我们证明褪黑素通过调节黏着斑和细胞外基质受体通路相关基因促进毛囊伸长，进一步对lncRNA靶基因的顺式预测表明褪黑素介导的lncRNA主要靶向血管平滑肌收缩和调节干细胞多能性的信号通路。我们认为褪黑激素暴露不仅干扰了毛囊干细胞分泌的调控毛囊发育的关键信号，而且介导了主要针对微血管系统和细胞外基质的lncRNA，这些微血管系统和细胞外基质构成了毛囊干细胞高度协调的微环境。总之，我们的研究结果提供了一个深刻的看法lncRNA在调节羊绒山羊毛囊昼夜节律和扩大我们的知识褪黑激素介导的毛囊形态变化。
ABSTRACT The role of melatonin in promoting the yield of Cashmere goat wool has been demonstrated for decades though there remains a lack of knowledge regarding melatonin mediated hair follicle growth. Recent studies have demonstrated that long non-coding RNAs (lncRNAs) are widely transcribed in the genome and play ubiquitous roles in regulating biological processes. However, the role of lncRNAs in regulating melatonin mediated hair follicle growth remains unclear. In this study, we established an in vitro Cashmere goat secondary hair follicle culture system, and demonstrated that 500 ng/L melatonin exposure promoted hair follicle fiber growth. Based on long intergenic RNA sequencing, we demonstrated that melatonin promoted hair follicle elongation via regulating genes involved in focal adhesion and extracellular matrix receptor pathways and further cis predicting of lncRNAs targeted genes indicated that melatonin mediated lncRNAs mainly targeted vascular smooth muscle contraction and signaling pathways regulating the pluripotency of stem cells. We proposed that melatonin exposure not only perturbed key signals secreted from hair follicle stem cells to regulate hair follicle development, but also mediated lncRNAs mainly targeted to pathways involved in the microvascular system and extracellular matrix, which constitute the highly orchestrated microenvironment for hair follicle stem cell. Taken together, our findings here provide a profound view of lncRNAs in regulating Cashmere goat hair follicle circadian rhythms and broaden our knowledge on melatonin mediated hair follicle morphological changes.